胡保奎, 耿召华, 祝善俊, 刘玉峰. 新生大鼠心肌细胞的原代培养及鉴定[J]. 心脏杂志, 2013, 25(6): 654-656.
    引用本文: 胡保奎, 耿召华, 祝善俊, 刘玉峰. 新生大鼠心肌细胞的原代培养及鉴定[J]. 心脏杂志, 2013, 25(6): 654-656.
    shu
    Culture and identification of cardiomyocytes in neonatal rats[J]. Chinese Heart Journal, 2013, 25(6): 654-656.
    Citation: Culture and identification of cardiomyocytes in neonatal rats[J]. Chinese Heart Journal, 2013, 25(6): 654-656.
    shu

    新生大鼠心肌细胞的原代培养及鉴定

    Culture and identification of cardiomyocytes in neonatal rats

      摘要
    • 摘要: 目的:探讨新生大鼠心肌细胞原代培养的方法，培养存活率高和纯度高的心肌细胞。方法:无菌条件下取出出生1~2 d的SD大鼠心脏，用2.5 g/L胰蛋白酶和 2.0 g/L Ⅱ型胶原酶等量混合液在37℃条件下分次消化心肌组织。以差速贴壁并将时间延长至90 min纯化心肌细胞，48 h后换液。结果： 将心肌细胞24 h贴壁生长，于2~3 d心肌细胞伸出伪足，形成细胞簇，同步搏动，频率约80~130次/min，存活率为(93.9±2.8)%，纯度为(91.9±2.2)%。结论:以本实验的方法培养的心肌细胞存活率高、纯度高，是一种较为理想的原代心肌细胞培养方法。

       

      Abstract: AIM:To develop a method of primary culture of neonatal rat cardiomyocytes with high survival cell rate and high purity. METHODS: Hearts of 1 to 2dayold neonatal Sprague Dawley rats were removed under sterile conditions with 0.25% trypsin and 02% collagenase type II at 37℃ under the conditions of graded digestion myocardial tissue. Differential adherence purification was applied. The time was extended to 90 min and the medium was changed after 48 h. RESULTS: Adherent growth of myocardial cells was observed at 24 h and pseudopodia growth at 2-3 days. Myocardial cells formed cell clusters and the synchronous beat frequency was about 80-130 times/min with survival rate of (93.9±2.8)% and purity of (91.9±2.2)%. CONCLUSION: We developed a method to culture myocardial cells with high survival cell rate and high purity.

       

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