Abstract: AIM: To investigate the biocompatibility of polylactic-co-glycolic acid (PLGA) and cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells (BMMSCs) so as to provide a basis for the study of myocardial tissue engineering and in vivo implantation. METHODS: BMMSCs were isolated from bone marrow of SD mice by density gradient centrifugation. BMMSCs of passage 3 were induced and differentiated to cardiomyocyte-like cells with 10 μmol/L 5-azacytidine (5-aza) and 0.1 μmol/L angiotensin II (AngII) in culture medium. Cardiomyocyte-like cells were then inoculated into the pores of PLGA pieces. The cell adhesion rate and the adhesive power were examined by conventional precipitation method and the proliferation behavior of the cells was analyzed by MTT assay. Scanning electron microscopy (SEM) was used to observe the morphology of cardiomyocyte-like cells. RESULTS: Cardiomyocyte-like cells attached, proliferated and secreted extracellular matrix in porous PLGA. No obvious significance was observed between PLGA group and cell-only group. CONCLUSIONS: Cardiomyocyte-like cells can adhere to PLGA and proliferate, indicating PLGA can be used as a scaffold in myocardial tissue engineering.