补肾活血汤对去卵巢大鼠心肌微血管和CYP2J3的影响

王玉珍; 刘海涛; 宋欢欢; 张玉芬; 马 静

补肾活血汤对去卵巢大鼠心肌微血管和CYP2J3的影响

Effect of Bushenuoxue decoction on myocardial microvascular vessels and CYP2J3 in ovariectomized rats

摘要

摘要: 目的观察补肾活血汤（AED）对去卵巢大鼠心肌中细胞色素P4502J3蛋白（CYP2J3）表达、心肌微血管数目和血清雌二醇（E2）水平的影响。方法 48只成年SD雌鼠随机分为8组：正常对照组（Control组）、假手术组(Sham组)、去卵巢组(OVX组)、AED高剂量组(AED-H组)、AED低剂量组(AED-L组)、17β-雌二醇干预组(OVX/ERT组)、雌激素受体拮抗剂ICI182780干预组（AED-H/FET组及AED-L/FET组）。各组大鼠在处死前进行血液流变性指标的检测；Q-PCR和Western blot检测心肌CYP2J3的表达变化，免疫组化染色法检测心肌微血管数目变化，心脏B超检测心肌血流动力学变化，放射免疫法检测血清E2水平改变。结果 OVX组心肌微血管计数明显下降（P<0.01），而OVX/ERT组与AED-H组心肌微血管计数改善明显；OVX组大鼠心肌CYP2J3 mRNA和蛋白表达明显下降，而OVX/ERT组与AED-H组的CYP2J3 mRNA和蛋白表达下降不明显；OVX组大鼠的全血黏度、血浆黏度及红细胞压积较Sham组大鼠增高，而OVX/ERT组及AED-H组全血黏度、血浆黏度及红细胞压积明显下降。OVX组大鼠的红细胞变形指数、红细胞刚性指数和红细胞电泳时间均明显增高（P<0.01），但在OVX/ERT组及AED-H组较OVX组大鼠明显下降（P<0.05）。OVX组血流动力学各项指标均降低（P<0.05），而与补充雌激素组相同，AED-H组左室射血分数改善明显。OVX组大鼠血清E2含量显著下降(P<0.05)，高剂量、低剂量AED均可使OVX组大鼠E2含量显著升高(P<0.05)。结论 AED能增加心肌微血管数目，改善心肌血流动力学，其作用可能与增加OVX大鼠循环雌激素水平及促进CYP2J3蛋白表达有关。

Abstract: AIM To observe the effects of bushenhuoxue decoction on myocardial blood flow dynamics, number of myocardial microvascular vessels, serum estradiol levels and myocardial CYP2J3 expression in ovariectomized rats. METHODS Forty-eight adult female Sprague Dawley rats were randomly divided into eight groups: normal group (NORM), sham group (SHAM), ovariectomized group (OVX), high-dose Bushenhuoxue decoction group (AED-H), low-dose Bushenhuoxue decoction group (AED-L), 17β-estradiol intervention group (OVX/ERT group), and ICI182780 intervention groups (AED-H/FET and AED-L/FET). Hemorheology was detected before rats were killed. Cardiac hemodynamic were detected by cardiac ultrasound instrument. The number of myocardial microvascular vessels was determined by immunohistochemical method. Serum estradiol levels were measured by radioimmunoassay, and cardiac CYP2J3 expressions were determined by Q-PCR and Western blot. RESULTS Blood flow dynamic indicators declined in OVX group compared to those in NORM group (P<0.05). Left ventricular ejection fraction improved significantly in both estrogen group and AED-H group. Serum E2 level decreased significantly in OVX group (P<0.05) but both high- and low-dose Bushenhuoxue decoction significantly promoted the serum E2 level in OVX group (P<0.05). In OVX group, whole blood viscosity, plasma viscosity and hematocrit increased compared to sham group, whereas in OVX/ERT group and AED-H group, these indexes decreased obviously. Red cell deformation time index, erythrocyte rigidity index and erythrocyte electrophoresis were significantly higher in the OVX group (P<0.01). However, these indexes significantly decreased in OVX/ERT group and AED-H group compared to OVX group (P<0.05). Myocardial microvascular counts in OVX group decreased significantly, whereas the myocardial microvascular count in estrogen group and AED-H group improved significantly (P<0.05). Similarly, myocardial CYP2J3 mRNA and protein expression in OVX group decreased significantly, whereas only a slight decrease was observed in estrogen group and AED-H group. CONCLUSION Bushenhuoxue decoction can increase the number of myocardial microvascular vessels and improve cardiac hemodynamics in ovariectomized rats. This effect may be mediated by increasing the level of circulating estrogen in ovariectomized rats and promoting CYP2J3 protein expression.

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