Abstract: AIM:To investigate the association between IL-8 +394T/G polymorphism and acute coronary syndrome (ACS) in a Han population in Northern China. METHODS: A case-control study was conducted in 675 patients with ACS and 636 controls with normal coronary angiograms. Polymorphic genotypes were determined by polymerase chain reaction and sequencing analysis. RESULTS: Genotype frequencies in IL-8 +394 T/G polymorphism were in good concordance with the Hardy-Weinberg equilibrium in both case and control groups. Genotype frequencies of GG, GT and TT of IL-8 +394 T/G polymorphism were 18.1%, 50.9% and 31.0% in the controls, and 16.9%, 48.7% and 34.4% in patients with ACS, respectively. There were no significant differences in the genotype and allele distribution of +394 T/G polymorphism of the IL-8 gene between groups (P>0.05). Logistic regression analysis with adjustments for other risk factors revealed that the IL-8 +394 T/G allele carriers did not significantly increase the risks of ACS compared with noncarriers (P>0.05). CONCLUSIONS: IL-8 +394 T/G polymorphism was not a genetic risk factor for ACS in a Han population in Northern China.