Abstract: AIM To observe expression, activation and recruitment of integrin changes and associated signal transduction molecules under different transmural pressures by combining simulated weightlessness treatment and vascular perfusion culture. METHODSWhole animal level: Effects of simulated weightlessness on the expressions of integrin αV, integrin β3, Src, ERK and p-ERK were observed. Blood vessel level: The pressure vessel culture system that could control the flow rate and pressure was established. RESULTSWestern blot analysis revealed that an increased expression of integrin αV, Src and p-ERK was observed by tail suspension. No significant difference in integrin β3 and ERK protein expression was detected. High pressure culture of 150 mmHg induced significant protein expression of integrin aV, integrin β3, Src, ERK and p-ERK in carotid artery. Daily 1-h normal pressure recovery of 80 mmHg partly restored the changes of integrin aV, integrin β3 and Src expression. CONCLUSIONRedistribution of transmural pressures may be the primary factor that initiates region-specific remodeling of arteries during microgravity. Daily 1-h normal pressure recovery partly prevents vascular remodeling and changes of integrin downstream molecules.