王德国, 赵 胜, 王 新, 张凤祥, 曹克将. 扩张性心肌病患者心房Cx43蛋白的表达及其意义[J]. 心脏杂志, 2012, 24(3): 340-343.
    引用本文: 王德国, 赵 胜, 王 新, 张凤祥, 曹克将. 扩张性心肌病患者心房Cx43蛋白的表达及其意义[J]. 心脏杂志, 2012, 24(3): 340-343.
    shu
    Connexin43 expression and distribution in left atrial tissue from patients with combined dilated cardiomyopathy and atrial fibrillation[J]. Chinese Heart Journal, 2012, 24(3): 340-343.
    Citation: Connexin43 expression and distribution in left atrial tissue from patients with combined dilated cardiomyopathy and atrial fibrillation[J]. Chinese Heart Journal, 2012, 24(3): 340-343.
    shu

    扩张性心肌病患者心房Cx43蛋白的表达及其意义

    Connexin43 expression and distribution in left atrial tissue from patients with combined dilated cardiomyopathy and atrial fibrillation

      摘要
    • 摘要: 目的:观察扩张性心肌病（dilated cardiomyopathy，DCM）患者心房连接蛋白43（Cx43）的表达、磷酸化的Cx43（pCx43）及其分布特征。探讨Cx43与房颤（atrial fibrillation，AF）之间的关系。方法： 18例接受心脏移植术的DCM患者中，8例无AF史且手术时为窦性心律（SR，SR组），6例有AF史但手术时为SR（AF+SR组），4例有AF史且手术时有AF（AF+AF组）。用超声心动图(ECG)测定左心房大小与左心室收缩功能；用免疫印迹法及免疫荧光染色法检测Cx43、pCx43及其分布。结果： DCM患者的左心房直径(LAD)、左室射血分数(LVEF)在SR、AF+SR、AF+AF组间均没有显著差异。与SR组相比，AF+SR组心房组织中Cx43和pCx43无显著差异；AF+AF组总Cx43的表达量及pCx43显著升高(P<0.01及P<0.05)。病理观察提示，AF+AF组Cx43和pCx43升高，Cx43向细胞两侧分布，且pCx43升高。结论： DCM患者的房颤节律伴随Cx43的表达、磷酸化及其分布异常。

       

      Abstract: AIM:To investigate Connexin 43 (Cx43) expression, phosphorylation and distribution in atrium of dilated cardiomyopathy (DCM) patients with or without atrial fibrillation (AF) and to demonstrate the relationship between Cx43 and AF. METHODS: Among 18 DCM patients who underwent heart transplantation, eight patients had sinus rhythm (SR) at the time of surgery (SR group) without AF history. Six patients maintained SR with AF history (AF+SR group) and four patients persisted with AF(AF+AF group). Echocardiography was performed to determine left atrial diameter (LAD) and cardiac function. Expressions of Cx43 and phosphorylated Cx43 at the site of Ser368 (pSer368Cx43) were detected by immunofluorescence staining and Western blot. RESULTS: No significant difference was observed in LAD and cardiac function among the three groups and no significant difference was seen in the level of Cx43 and pSer368Cx43 between SR group and AF+SR group. In contrast, total Cx43 (TCx43) in AF+AF group was obviously higher than in the SR group and AF+SR group. Phosphorylated Cx43 (pCx43) increased in atrial tissue of AF+AF group and redistributed from intercalated disk to the lateral borders of myocytes. CONCLUSIONS: AF rhythm in DCM may be accompanied by abnormalities in atrial Cx43 expression, phosphorylation and distribution.

       

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