Salusin-β对缺血/再灌注心肌损伤的影响

王键飞; 周旭; 印媛君; 袁文俊; 林丽; 任安经

Salusin-β对缺血/再灌注心肌损伤的影响

Effects of salusin-β on heart ischemia-reperfusion injury

摘要

摘要: 目的: 探明salusin-β——一种新发现的心血管调节肽是否具有抗心肌缺血/再灌损伤(RMMI)的作用。方法: 以离体灌流大鼠的心脏RMMI为模型，分为对照组和salusin-β预处理组，观察各组大鼠心脏缺血/再灌注(I/R)后的心率(HR)、冠脉流量(CF)和心功能［左室收缩压(LVSP)、左室舒张末压(LVEDP)和左室内压上升、下降速率（±dp/dt）］的变化。结果: 以10-8 mol/L的salusin-β预处理，可以改善心脏I/R后CF、LVDP、LVEDP、+dp/dt和-dp/dt等指标。10-8 mol/L salusin-β组复灌30 min时，CF、LVDP、+dp/dt和-dp/dt的恢复率，分别是停灌前的（52±5）%、（79±8）%、（70±8）%和（69±10）%，显著高于对照组的（45±6）%、（68±10）%、（58±11）%和（57±8）%（P<0.01，n=8）。此外，10-8 mol/L salusin-β组复灌30 min时，LVEDP为(28.25±7.62)mmHg，显著低于对照组的(52.52±11.27)mmHg。结论: 上述结果表明，salusin-β具有抗心肌RMMI、改善I/R后心脏功能的作用。

Abstract: AIM: To investigate whether salusin-β, a new regulatory peptide relevant to the cardiovascular system, can alleviate heart ischemia-reperfusion injury. METHODS: The effects of salusin-β on heart rate (HR), coronary flow (CF) and heart function (LVSP, LVEDP and ±dp/dt) were observed in isolated rat hearts after ischemia-reperfusion. RESULTS: Treatment with 10-8 mol/L salusin-β significantly improved the post-ischemic CF, LVDP, LVEDP and ±dp/dt compared with those of the untreated hearts. The recovery of CF, LVDP, +dp/dt and dp/dt after reperfusion for 30 min was, respectively, (52±5)%, (79±8)%, (70±8)% and (69±10)% vs. (45±6)%, (68±10)%, (58±11)% and (57±8)% in control group (P<0.01, n=8). In addition, in the presence of 10-8 mol/L salusin-β, LVEDP (28.25±7.62)mmHg improved significantly compared with that in control group (52.52±11.27)mmHg. CONCLUSION: These results show that salusin-β alleviates ischemia-reperfusion injury and improves heart function.

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