Abstract: AIM:To evaluate the severity of myocardial damage after sleep deprivation and to explore its mechanism. METHODS: Sixty rats were randomized into six groups (ten in each group) and sleep deprivation rat models were established by “multi-platform method” (MMPM). Effects of sleep deprivation on the contents of ischemia modified albumin (IMA), high-sensitivity C-reactive protein (hs-CRP), glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) in rat myocardium were evaluated. RESULTS: Compared with those in the normal cage control (CC) and tank control (TC) group, the contents of IMA, hs-CRP, GSH and MDA increased (P<0.05). With the elongation of sleep deprivation time, the contents of these markers increased, but the activity of SOD showed a decreasing tendency. CONCLUSION: Sleep deprivation contributes to rat myocardial oxidative stress and inflammation. With the aggravating oxidative stress and inflammation, the damaging effects of ischemia and hypoxia on myocardium become more serious.