朱元州, 冯义柏, 常超, 王裕勤. 去甲肾上腺素预处理晚期出现的线粒体跨膜电位的变化[J]. 心脏杂志, 2009, 21(6): 779-781.
    引用本文: 朱元州, 冯义柏, 常超, 王裕勤. 去甲肾上腺素预处理晚期出现的线粒体跨膜电位的变化[J]. 心脏杂志, 2009, 21(6): 779-781.
    shu
    Change of transmembrane potential during delayed phase of noradrenaline preconditioning[J]. Chinese Heart Journal, 2009, 21(6): 779-781.
    Citation: Change of transmembrane potential during delayed phase of noradrenaline preconditioning[J]. Chinese Heart Journal, 2009, 21(6): 779-781.
    shu

    去甲肾上腺素预处理晚期出现的线粒体跨膜电位的变化

    Change of transmembrane potential during delayed phase of noradrenaline preconditioning

      摘要
    • 摘要: 目的: 研究微量去甲肾上腺素(NA)预处理晚期心肌细胞线粒体跨膜电位的变化，为探讨预适应晚期作用机制提供依据。方法: 原代培养乳鼠心肌细胞随机分为3组，即对照（control,CON）组，缺氧处理（hypoxia treatment,HT）组和NA预处理 (noradrenaline preconditioning,NAPC)组。CON组：不给任何药物干预，也不进行HT，培养24 h；HT组：不给予任何药物干预，培养24 h后直接进行HT 40 min；NAPC组：预先给予终浓度为0.2 mg/L的NA孵育10 min后，继续培养24 h再进行HT 40 min。用流式细胞仪检测各组罗丹明123（rhodamine 123）标记的线粒体的荧光强度（fluorescence intensity,FI）。结果: 3组的FI值通过方差分析比较（F=355.5,P<0.05），HT组与NAPC组的FI比CON组明显降低［（6.24±0.13）%、（6.68±0.22）% vs （8.32±0.19）%,P<0.05）］，表明HT组与NAPC组心肌细胞线粒体的跨膜电位明显降低，细胞能量代谢能力下降；NAPC组FI比HT组高［(6.68±0.22)% vs （6.24±0.13）%，P<0.05)］，表明NAPC组心肌细胞线粒体的跨膜电位较高，NA预处理过的细胞能量代谢能力较强。结论: NAPC组的心肌细胞线粒体的FI较HT组明显升高，表明NA预处理晚时期跨膜电位降较高，NA触发了细胞保护效应，使心肌细胞对损伤性刺激的耐受力增强。

       

      Abstract: AIM: To investigate the effect of noradrenaline (NA) on transmembrane potential during delayed preconditioning phase. METHODS: Primary culture myocardial cells of neonatal rat were randomly divided into three groups, namely, control (CON) group, hypoxia treatment (HT) group, and NA preconditioning (NAPC) group. CON group was cultured for 24 h without any drug interference, HT group was cultured for 24 h without any drug interference but then treated for 40 min with hypoxia, and NAPC group was exposed to NA (0.2 mg/L) for 10 min and then treated with hypoxia for 40 min after the 24-h culture. Fluorescence intensity (FI) of rhodamine-123-marked mitochondria was detected by flow cytometry. RESULTS: FI of the three groups was examined by analysis of variance (F=355.5, P<0.05). FIs of HT group and NAPC group were lower than that of CON group ［(6.24±0.13)%, (6.68±0.22)% vs. (8.32±0.19)%, P<0.05］. Transmembrane potentials of HT group and NAPC group were lower than that of CON group, indicating that the energy synthesis was defective. FI of NAPC group was higher than HT group and the transmembrane potential of NAPC group was higher than HT group, indicating that NA increased energy synthesis and improved cardiomyocyte metabolism. CONCLUSION: FI of NAPC group is higher than HT group, which indicates that NA improves the transmembrane potential.

       

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