李 佳, 葛海龙, 陈光远, 高倩萍, 孙俊峰, 李元十, 富 路. 房颤对外周血CD34+造血祖细胞的影响及SDF-1/CXCR4在心房中的表达[J]. 心脏杂志, 2011, 23(5): 608-612.
    引用本文: 李 佳, 葛海龙, 陈光远, 高倩萍, 孙俊峰, 李元十, 富 路. 房颤对外周血CD34+造血祖细胞的影响及SDF-1/CXCR4在心房中的表达[J]. 心脏杂志, 2011, 23(5): 608-612.
    shu
    Effect of atrial fibrillation on human CD34+ hematopoietic progenitor cells in circulation and expression of SDF-1/CXCR4 in atrium[J]. Chinese Heart Journal, 2011, 23(5): 608-612.
    Citation: Effect of atrial fibrillation on human CD34+ hematopoietic progenitor cells in circulation and expression of SDF-1/CXCR4 in atrium[J]. Chinese Heart Journal, 2011, 23(5): 608-612.
    shu

    房颤对外周血CD34+造血祖细胞的影响及SDF-1/CXCR4在心房中的表达

    Effect of atrial fibrillation on human CD34+ hematopoietic progenitor cells in circulation and expression of SDF-1/CXCR4 in atrium

      摘要
    • 摘要: 目的:研究不同类型的房颤(AF)对人外周血CD34+造血祖细胞（CD34+HPCs）的影响，以及持续心房快速起搏犬心肌基质细胞衍生因子-1（SDF-1）及其受体CXCR4的表达，初步探讨CD34+HPCs及SDF-1/CXCR4在AF时心肌损伤修复中的作用。方法： 应用流式细胞术测定阵发性AF患者组(n=35)、持续性AF患者组(n=35)及窦性心律者对照组(n=30)外周血中CD34+HPCs的百分含量；并对持续性AF患者组中24例患者成功进行体外直流电复律后48 h，测定外周血中CD34+HPCs的百分含量。另外，将成年健康杂种犬13条随机分为两组：即快速起搏组(n=7)和假手术组(n=6)，均开胸后于右心耳缝植AOO型起搏器，快速起搏组以400次/min起搏6周，假手术组不起搏。应用RT-PCR测定左心耳和左心房CXCR4 mRNA的表达水平，用蛋白质免疫印迹法检测左心房SDF-1蛋白的表达。结果： 持续性AF患者组外周血中CD34+HPCs的百分含量明显高于阵发性AF患者组和对照组（P<0.05）；而后两组间无差别。持续性AF患者成功进行体外直流电复律后48 h，外周血中CD34+HPCs的百分含量较复律前明显下降（P<0.05）。快速起搏组犬左心耳和左心房CXCR4 mRNA表达的水平明显高于假手术组（P<0.05），左心耳增高16.7%，左心房增高18.8%；SDF-1蛋白质表达的水平亦明显高于假手术组（P<0.01）。结论： 持续性AF患者外周血中CD34+HPCs的数量增加；心房快速起搏犬心房SDF-1/CXCR4的表达增加。CD34+HPCs和SDF-1/CXCR4可能参与了持续性AF患者心房损伤时心肌组织的修复过程。

       

      Abstract: AIM:To investigate the effect of different kinds of atrial fibrillation (AF) on human CD34+ hematopoietic cells (HPCs) in circulation and on myocardial expression of SDF-1 and its receptor CXCR4 in canines with lasting rapid atrial pacing and to explore the role of CD34+ HPCs and SDF-1/CXCR4 in repairing atrium during AF. METHODS: Included in our study were 100 subjects (35 with paroxysmal AF, 35 with persistent AF and 30 with sinus rhythm). Percentages of CD34+ HPCs in peripheral venous blood were tested by flow cytometry. Percentages of CD34+ HPCs were tested again at 48 h after receiving successful direct current cardioversion in 24 subjects with persistent AF. Thirteen canines were randomly divided into two groups: sham-operated group (n=6) and atrial pacing group (n=7). A pacemaker (AOO) was implanted to the right atrial appendage in each of the dogs. Dogs in the atrial pacing group were paced at 400 bpm for 6 weeks, whereas those in the sham-operated group were not paced. At the end of the study, expression of CXCR4 at mRNA level in myocardium of left atrium and left auricle was analyzed by reverse transcription polymerase chain reaction. SDF-1 protein in left atrium of each group was examined using Western blot. RESULTS: Subjects with persistent AF had higher level of CD34+ HPCs than those in patients with paroxysmal AF and sinus rhythm (P<0.05), with no difference between the latter two groups. The level of CD34+ HPCs in persistent AF decreased after successful cardioversion (P<0.05). Compared with the sham-operated group, lasting rapid atrial pacing significantly increased the expression of CXCR4 at mRNA level in left atrium and left auricle (P<0.05) and the expression of SDF-1 protein also increased (P<0.01). CONCLUSION: The percentage contents of CD34+ HPCs in peripheral venous blood in persistent AF increases and lasting rapid atrial pacing significantly increases the expression of SDF-1 and CXCR4 in atrium. SDF-1/CXCR4 may be involved in the process of myocardium repair during the atrium injuries caused by persistent AF.

       

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