Abstract: AIM:To investigate the establishment and management of anesthesia and cardiopulmonary bypass (CPB) in a mini-pig model. METHODS: Twelve mini-pigs were used in this experiment. After successful anesthesia and tracheal intubation, mediastinotomy was performed to expose the heart and great vessels. CPB was established with cannulations of ascending aorta as well as superior and inferior vena cava. The circuit was composed of a mini-type membrane oxygenator, an arterial filter and a roller pump, which was primed with 250 ml balanced solution. CPB was conducted under moderate hypothermia and hearts were arrested with antegrade blood cardioplegia infusion. Hemodynamic data, arterial and venous oxygen saturation (SaO2, SvO2), hematocrit (Hct) and blood lactate acid concentrations (Lac) were determined at different time points. RESULTS: The blood was moderately diluted according to Hct values. SaO2 and SvO2 were maintained at the normal range during the CPB period, whereas Lac increased significantly with elapsed CPB time. Mean arterial blood pressure decreased significantly compared with the pre-bypass value and returned to baseline value after CPB disconnection. All hearts were successfully arrested with cardioplegia infusion and spontaneous rhythm restored upon aortic cross-clamping removal. CONCLUSION: CPB is successfully established in this mini-pig model, which can be used for CPB-related in vitro experiments.s and R219K variant significantly modulates the HDL-C response to pravastatin in patients with AMI.