Abstract: AIM: To analyze the sensitivity and specificity of adenosine stress myocardial perfusion tomographic imaging in the diagnosis of coronary artery diseases (CAD). METHODS: Adenosine was infused IV at a rate of 0.14 mg/(kg·min) and 3 min after adenosine infusion, 740 MBq of 99mTc-MIBI was injected IV. Single photon emission computer tomography (SPECT) myocardial imaging acquisition was obtained 1.5 h after adenosine infusion. With an abnormal result, rest myocardial perfusion imaging would be performed on the next day. Coronary angiography was performed in all patients within 1 week of myocardial imaging. RESULTS: Sixty cases (57±11 years old), 35 men, 25 women were included in this study. In the 50 cases of CAD patients confirmed by coronary angiography, 42 patients had positive adenosine stress myocardial perfusion tomographic imaging. Thirteen of the 18 cases without CAD had negative adenosine stress myocardial perfusion tomographic imaging. Sensitivity and specificity of adenosine myocardial perfusion tomographic imaging in the diagnosis of CAD were 88% and 72%, respectively. Sensitivity of adenosine myocardial perfusion tomographic imaging in the diagnosis of coronary stenosis in left anterior descending, left circumflex and right coronary artery were 20/36, 14/22 and 27/28, respectively. No severe adverse side effect was observed during adenosine stress test. CONCLUSION: Adenosine stress myocardial perfusion imaging has relatively high sensitivity and specificity in the diagnosis of CAD and plays an important role in the diagnosis of CHD.