卢琳; 贾如意

Relationship between serum nuclear factor-κB and serum inflammatory cytokines levels in heart failure following acute myocardial infarction in rats

摘要

摘要: 目的:探讨急性心肌梗死后（AMI）心衰(HF)大鼠血清核因子-κB（NF-κB）的水平与肿瘤坏死因子-α（TNF-α）、高敏C反应蛋白（hsCRP）分泌的相关性。方法：将50只SD大鼠随机分为3组：HF组（20只）、治疗组（20只）和假手术组（10只）。结扎大鼠冠状动脉前降支建立AMI后HF模型。治疗组在手术结扎后饮水中给予NF-κB抑制剂吡咯烷二硫代氨基甲酸盐(pyrrolidine dithiocarbamate，PDTC)，HF组和假手术组给予同等量的蒸馏水。术后6周，行血流动力学检测；秤取心脏组织的湿质量；取动脉血分离血清，用ELISA法检测NF-κB、TNF-α的水平，用乳胶增强透射免疫比浊法检测hsCRP水平。结果： ①HF组和治疗组大鼠心室重构指数和肺指数明显高于假手术组（P<0.05）。②HF组和治疗组大鼠血流动力学的状况较假手术组明显下降（P<0.05），但治疗组较HF组有所好转（P<0.05）。③HF组和治疗组血清NF-κB、TNF-α和hsCRP的水平较假手术组明显升高（P<0.05）；治疗组又较HF组明显下降（P<0.05）。④NF-κB的水平与TNF-α、hsCRP的水平呈显著的正相关（r分别为0.465和0.323，均P<0.05）。结论： AMI后HF大鼠血清NF-κB的水平升高，炎性细胞因子TNF-α和hsCRP分泌增多，HF大鼠可能是通过NF-κB水平的升高上调炎性细胞因子的分泌。

Abstract: AIM:To study the relationship of serum nuclear factor-κB (NF-κB) and the level of serum TNF-α and high-sensitivity C-reactive protein (hsCRP) in heart failure following acute myocardial infarction (MI) in rats. METHODS: A total of 50 SD rats were randomized into three groups: heart failure group (n=20), treatment group (n=20) and sham-operated group (n=10). Heart failure model was created in rats by coronary artery ligation after MI. After ligation, NF-κB inhibitor pyrrolidine dithiocarbamate was given in drinking water in treatment group and the same amount of distilled water was given in heart failure group and sham-operated group. All surviving models were sacrificed after 6 weeks. Hemodynamic parameters and ventricular mass index were measured. Serum NF-κB and TNF-α levels were detected using ELISA and hsCRP was detected using latex-enhanced immunoturbidimetric assay transmission. RESULTS: Compared with sham-operated group, ventricular remodeling index and lung index in heart failure group and treatment group significantly increased (P<0.05). Compared with heart failure group, indexes in treatment group decreased but were still significantly higher than in sham-operated group (P<0.05). Hemodynamic status significantly decreased in heart failure group and treatment group compared with sham-operated group (P<0.05) and status in the treatment group was superior to that in the heart failure group (P<0.05). Serum NF-κB, TNF-α and hsCRP levels in heart failure group and treatment group significantly increased compared with sham-operated group (P<0.05). Levels in treatment group significantly decreased compared with heart failure group (P<0.05). NF-κB levels were positively correlated with levels of TNF-α (r=0.465, P<0.05) and levels of hsCRP (r=0.323, P<0.05). CONCLUSION: Levels of serum NF-κB and serum TNF-α and hsCRP in heart failure following acute MI in rats increased, suggesting that NF-κB upregulates the levels of inflammatory cytokines in heart failure rats.

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