Abstract: AIM:To study the protection due to guanxinkeli (GXKL) on myocardial ischemic and reperfusion injury. METHODS: Thirty six rats were randomly divided into three groups: control, ischemia reperfusion injury, and resveratrol. Rat hearts were subjected to 15 min ischemia and then followed by 30 min reperfusion. HR, LVDP, LVEDP and ±dp/dtmax were recorded before ischemia and at 30 min of reperfusion. Coronary effulent was collected before ischemia and at 30 min of reperfusion to measure the CK and LDH activity. The arrhythmia scores were determined. RESULTS: GXKL enhanced the LVEDP, LVDP and ±dp/dtmax. The arrhythmia score, CK and LDH activity were lower in the GXKL group than in ischemia and reperfusion group. JNK activity was also lower in the GXKL group. CONCLUSION: GXKL has protected isolated myocardial ischemia and reperfusion injury. The JNK signaling pathway may play an important role in the mechanism.