普罗布考对糖基化终末产物引发心脏微血管内皮功能紊乱的作用

Effect of probucol on advanced glycosylation end product-induced dysfunction in cardiac microvascular endothelial cells

摘要: 目的:观察普罗布考（probucol）对糖基化终末产物（AGEs）作用后的心脏微血管内皮细胞iNOS表达的影响。方法：原代培养心脏微血管内皮细胞，AGEs（100 mg/L）体外模拟高糖环境，在probucol(5 μmol/L,10 μmol/L,20 μmol/L) 作用后，检测ROS、NO和iNOS变化情况。结果：与AGEs组比较，probucol组中ROS蛋白表达降低，NO生成增加，而 iNOS蛋白表达降低，且显示有浓度依赖性。结论： probucol可能通过对抗氧化应激的途径，缓解AGEs引发的心脏微血管内皮功能障碍。

Abstract: AIM:To observe the effect of probucol on the iNOS expression induced by advanced glycosylation end products (AGEs) in cardiac microvascular endothelial cells. METHODS: Primary cardiac microvascular endothelial cells were cultured in vitro. After treatment with AGEs (100 mg/L) and probucol (5 μmol/L, 10 μmol/L, 20 μmol/L), NO, iNOS and ROS protein expressions in all groups were detected and compared between groups. RESULTS: Compared with AGEs group, NO and iNOS protein expression increased and ROS protein expression decreased in groups treated with probucol. CONCLUSION: Probucol is able to partially recover AGE-induced dysfunction in cardiac microvascular endothelial cells.