急性冠脉综合征诱导单核-巨噬细胞中Notch信号受体1及炎性细胞因子的表达

付文波; 丁世芳; 李大主; 何少林; 王 妍; 黎 明; 冯义柏

急性冠脉综合征诱导单核-巨噬细胞中Notch信号受体1及炎性细胞因子的表达

Expression of Notch1 signaling and inflammatory cytokines in peripheral blood mononuclear cells of patients with acute coronary syndrome

摘要

摘要: 目的:探讨在急性冠脉综合征（acute coronary syndrome，ACS）患者外周血单核-巨噬细胞中Notch1分子及炎性细胞因子的表达。方法：以密度梯度离心法分离36例ACS患者、14例稳定型心绞痛患者及30例健康对照者外周血单核细胞，并以氟波酯（PMA）使之转化为巨噬细胞。分别采用实时（RT）-PCR和Western blot测定巨噬细胞中Notch1 mRNA和其蛋白的表达；采用RT-PCR和ELISA测定炎性细胞因子血管细胞黏附分子-1(VCAM-1) mRNA和其蛋白及单核趋化蛋白-1(MCP-1) mRNA和其蛋白的表达。结果：与对照组比较，ACS患者巨噬细胞中Notchl mRNA和其蛋白的表达明显升高(P<0.05)，ACS诱导的VCAM-1和MCP-1 mRNA和二者的蛋白浓度明显增高(P<0.05)。结论： Notch1信号通路及其介导的巨噬细胞天然免疫应答在动脉粥样硬化易损斑块的发生发展中发挥着重要的调节作用。

Abstract: AIM:To explore the differential expression of Notch signaling in human platelet-monocyte aggregates (PMAs) of patients with acute coronary syndrome (ACS). METHODS: PMAs of 50 patients with coronary artery disease (CAD) including 36 patients with ACS and 14 patients with stable angina (SA) were measured. Thirty subjects without CAD served as controls. After 48 h, cells and supernatant were collected separately. Expressions of Notch1 mRNA were measured by real-time quantitative PCR (RT-qPCR) and protein was measured by Western blot. Levels of vascular cell adhesive molecule-1 (VCAM-1) and monocyte chemoattractant protein-1 (MCP-1) in supernatant were determined by ELISA. RESULTS: Notch1 mRNA and protein expressions in ACS group were significantly higher than in controls (P<0.05). Levels of VCAM-1 and MCP-1 significantly increased in the CAD group (P<0.05). CONCLUSION: Notch1 and Notch1-mediated innate immune response may play an important regulatory role in the development and progression of atherosclerosis.

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