针灸预处理对心肌缺血/再灌注大鼠细胞凋亡及相关基因表达的影响

赵宇辉; 孙忠人

针灸预处理对心肌缺血/再灌注大鼠细胞凋亡及相关基因表达的影响

Effect of acupuncture and moxibustion preconditioning on cell apoptosis and correlative gene expression in myocardial ischemia/reperfusion injury in rats

摘要

摘要: 目的: 探讨针灸预处理对心肌缺血/再灌注损伤大鼠细胞凋亡及hsp70 mRNA表达的影响。方法: 利用大鼠心肌缺血/再灌注模型，观察针灸预处理组间（分为手针、电针和艾灸日1次组和日2次组），并与缺血预处理、单纯模型组、假手术组和正常对照比较大鼠细胞凋亡及hsp70 mRNA表达的情况。结果: 针灸预处理组均能降低细胞凋亡，促进hsp70 mRNA的表达，且与针灸预处理的次数有相关性。结论: 针灸预处理日1次组与缺血预处理预防心肌缺血/再灌注损伤的效应无显著差异，针灸预处理日2次组优于缺血预处理组。手针、电针和艾灸的效应接近，其效应皆与日次数有关。

Abstract: AIM: To explore the effect of acupuncture and moxibustion preconditioning on cell apoptosis and hsp70 mRNA in rat myocardial ischemia/reperfusion injury.METHODS: We used differentiating acupuncture and moxibustion preconditioning between groups and compared acupuncture and moxibustion preconditioning with ischemic preconditioning. The rats under study were divided into normal control group, pseudo-surgical group, ischemia/reperfusion group, ischemia preconditioning group, daily acupuncture preconditioning group, daily electroacupuncture preconditioning group, daily moxibustion preconditioning group, twice daily acupuncture preconditioning group, twice daily electroacupuncture preconditioning group, and twice daily moxibustion preconditioning group. RESULTS: Acupuncture and moxibustion preconditioning increased hsp70 mRNA expression and reduced cell apoptosis. Twice daily acupuncture and moxibustion preconditioning groups achieved better effects than ischemic preconditioning. CONCLUSION: Twice daily acupuncture and moxibustion preconditioning is more effective in preventing myocardial ischemia/reperfusion injury in rats.

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