张海军, 崔龙彪, 岳 原, 杜 成, 常耀明, 史 娟, 谢满江. STZ诱导的2型糖尿病大鼠心肌组织TRPC7的表达上调[J]. 心脏杂志, 2014, 26(4): 403-407.
    引用本文: 张海军, 崔龙彪, 岳 原, 杜 成, 常耀明, 史 娟, 谢满江. STZ诱导的2型糖尿病大鼠心肌组织TRPC7的表达上调[J]. 心脏杂志, 2014, 26(4): 403-407.
    shu
    Expression of cardiac TRPC7 channels increases in streptozotocin-induced diabetic rats[J]. Chinese Heart Journal, 2014, 26(4): 403-407.
    Citation: Expression of cardiac TRPC7 channels increases in streptozotocin-induced diabetic rats[J]. Chinese Heart Journal, 2014, 26(4): 403-407.
    shu

    STZ诱导的2型糖尿病大鼠心肌组织TRPC7的表达上调

    Expression of cardiac TRPC7 channels increases in streptozotocin-induced diabetic rats

      摘要
    • 摘要: 目的:观察2型糖尿病（T2DM）大鼠心肌组织损伤过程中，TRPC7的表达变化。方法: 以高脂饲料喂养并辅以腹腔注射小剂量链脲佐菌素（Streptozotocin,STZ）来建立T2DM大鼠模型，采用蛋白印迹及免疫组化染色方法检测TRPC7在T2DM大鼠心肌组织的表达变化。结果: ①T2DM大鼠造模成功且T2DM大鼠心肌组织发生损伤性变化：与正常组大鼠相比，T2DM组大鼠血糖明显升高且体重显著降低(P<0.05)；T2DM组大鼠心脏体积与质量显著减小(P<0.05)；T2DM组大鼠心脏质量指数(heart weight index,HWI)明显高于正常组(P<0.05)；②在T2DM大鼠心肌损伤过程中TRPC7表达上调：TRPC7在正常及T2DM大鼠心肌组织中均有表达，但与正常大鼠相比，T2DM大鼠心肌组织TRPC7蛋白的表达显著增高（P<0.05）。结论: 首次证实在损伤的T2DM大鼠心肌组织中，TRPC7的表达显著上调。

       

      Abstract: AIM:To investigate the change of cardiac TRPC7 expression in streptozotocin (STZ)-induced type 2 diabetic rats. METHODS: STZ-induced type 2 diabetic rat models were established and the expression of cardiac TRPC7 channels was evaluated by immunofluorescence and Western blot. RESULTS: 1) The impaired heart was found in STZ-induced T2DM rats. 2) The expression of cardiac TRPC7 significantly increased in STZ-induced T2DM rats as compared with that in control rats. CONCLUSION: The present work makes a novel finding that the expression of cardiac TRPC7 channels significantly increased in the impaired hearts of STZ-induced type 2 diabetic rats.

       

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