血浆钙调神经磷酸酶水平与左室舒张功能超声参数的相关性

付明强; 韩雪婷; 王艳艳; 张敬敬; 周京敏

血浆钙调神经磷酸酶水平与左室舒张功能超声参数的相关性

Relationship between plasma calcineurin level and echocardiographic parameters of left ventricular diastolic function

摘要

摘要: 目的探讨血浆钙调神经磷酸酶(calcineurin，CaN)水平与左室舒张功能超声参数的相关性。方法 6月龄自发性高血压大鼠(SHR)和Wistar大鼠各15只，采用ELISA方法检测血浆CaN和N末端脑钠肽前体（NT-proBNP）水平，通过小动物超声仪检测心脏舒张和收缩功能，分析血浆CaN水平与舒张功能超声参数的相关关系。结果与Wistar大鼠相比，6月龄SHR收缩压显著升高［(114±10)mmHg vs.(186±14)mmHg,P<0.01］；血浆CaN水平和NT-proBNP水平均高于Wistar大鼠［(1.2±0.4)ng/ml vs.(2.1±0.5)ng/ml，P<0.01；(229±85)fmol/ml vs.(327±86)fmol/ml，P<0.01)；6月龄SHR舒张功能超声参数和Wistar大鼠相比有统计学差异(E/A：2.5±0.4 vs 1.9±0.6；E′/A′：1.39±0.17 vs. 0.82±0.47；均P<0.01)。多元线性回归分析表明CaN与NT-proBNP、E/A、E′/A′具有相关性。线性相关分析表明，SHR大鼠血浆CaN水平与体质量、收缩压、NT-proBNP水平和等容舒张时间正相关(分别r=0.688，P<0.01；r=0.758，P<0.01；r=0.983，P<0.01；r=0.577，P<0.05)，与 E/A、E′/A′负相关(分别r=-0.977，P<0.01；r=-0.929，P<0.01)，与心率、射血分数、E/E′、减速时间不相关。结论血浆CaN水平升高与左室舒张功能不全具有相关性。

Abstract: AIM To investigate the relationship between plasma calcineurin level and echocardiographic parameters of left ventricular diastolic function. METHODS Plasma calcineurin and NT-proBNP levels were determined by ELISA assay in 15 male spontaneously hypertensive rats (SHR) and 15 six month old Wistar rats, cardiac diastolic and systolic functions were assessed by high resolution echocardiography with a 17.5 MHz probe. The relationships between plasma calcineurin and diastolic echocardiographic parameters were analyzed by correlation analysis and multiple linear regression analysis. RESULTS Compared to six-month-old Wistar rats, systolic blood pressure of SHR was higher than 140 mmHg ［(114±10) mmHg vs.(186±14) mmHg, P<0.01］. Plasma calcineurin and NT-proBNP levels both increased in SHR than those in Wistar rats ［(2.1±0.5) ng/ml vs.(1.2±0.4) ng/ml, P<0.01; (327±86) fmol/ml vs.(229±85) fmol/ml, P<0.01］. Further, E/A ratio and E′/A′ ratio significantly decreased in the SHR group compared with those of the Wistar rats (1.9±0.6 vs. 2.5±0.4 for E/A, 0.82±0.47 vs. 1.39±0.17 for E′/A′, both P<0.01). In a multiple linear regression model including body weight, systolic blood pressure, heart rate, ejection fraction, plasma NT-proBNP level, E/A, E′/A′, E/E′, deceleration time and isovolumic relaxation time, plasma NT-proBNP level, E/A and E′/A′ were independent variables of plasma calcineurin level (standardized coefficient=0.003, P<0.01; standardized coefficient=-0.354, P<0.01; standardized coefficient=-0.135, P<0.05, respectively). Linear correlation analysis showed plasma calcineurin level positively correlated with body weight, systolic blood pressure, plasma NT-proBNP level and isovolumic relaxation time (r=0.688, P<0.01; r=0.758, P<0.01; r=0.983, P<0.01; r=0.577, P<0.05, respectively). Plasma calcineurin level negatively correlated with E/A and E′/A′ (r=-0.977, P<0.01; r=-0.929, P<0.01), while there were no correlations between plasma calcineurin level and heart rate, ejection fraction, E/E′ as well as deceleration time. CONCLUSION Plasma calcineurin level in SHR is higher than that in Wistar rats and correlated well with diastolic echocardiographic parameters, suggesting plasma calcineurin level may be a useful biomarker for assessing left ventricular diastolic dysfunction.

HTML全文

参考文献(0)

施引文献

资源附件(0)