罗格列酮对血管紧张素Ⅱ诱导高血压大鼠的血压影响及其机制

邹晓译; 张 扬; 张双月; 刘双江; 王雪青

罗格列酮对血管紧张素Ⅱ诱导高血压大鼠的血压影响及其机制

Effect of rosiglitazone on angiotensin II-induced hypertension and its mechanism in rats

摘要

摘要: 目的:探讨罗格列酮（RSG）对血管紧张素Ⅱ(AngⅡ)诱导高血压大鼠血压的影响及机制。方法: 选择24只SD大鼠随机分为4组:正常对照组、RSG组、AngⅡ组及AngⅡ+RSG组。每组6只大鼠（n=6）。采用alzet渗透泵持续皮下泵入AngⅡ［300 ng/(kg·min)×7 d］建立高血压大鼠模型，RSG组和AngⅡ+RSG组给予RSG灌胃［5 mg/(kg·d)］7 d，7 d后观察各组大鼠的血压、心脏质量指数、空腹血糖变化,测定大鼠主动脉NADPH氧化酶的活性及超氧阴离子的含量。结果: 与AngⅡ组对比，AngⅡ+RSG组血压下降［(136±6) mmHg vs.(166±6) mmHg，P<0.01］及心脏质量指数下降［(3.54±0.04) mg/kg vs.( 3.85±0.08) mg/kg,P<0.01］；NADPH氧化酶活性及血管超氧阴离子含量下降［(288.49±36.19) cpm/μg vs.(584.04±69.67) cpm/μg，P<0.01；(2 792.82.7±726.76) cpm/mg vs.(4 765.50±597.34) cpm/mg，P<0.01］。结论: RSG抑制NADPH氧化酶的活性,降低血管超氧阴离子的含量,拮抗血管AngⅡ诱导的血压升高及心肌肥厚,发挥保护心血管的作用。

Abstract: AIM:To study the effects of rosiglitazone on angiotensin II (AngII)-induced hypertension in rats and to explore the associated mechanism. METHODS: Twenty-four Sprague Dawley rats were divided equally into four groups: control group, rosiglitazone group, AngII group, and AngII+rosiglitazone group. Rosiglitazone ［5 mg/(kg·d)］ was administrated with AngII ［(300 ng/(kg·min) s.c., osmotic pump］ for 7 days in the rats. Rosiglitazone was also given in sham-operated rats. Blood pressure, heart weight index values, vascular superoxide anion production and NADPH oxidase activity were determined. RESULTS: Compared with those in AngII group, blood pressure, heart weight index values, vascular superoxidase production and NADPH oxidase activity all decreased in AngII+rosiglitazone group ［(136±6) mmHg vs.(166±6) mmHg; (3.54±0.04) mg/g vs.(3.85±0.08) mg/g; (288.49±36.19) cpm/μg vs.(584.04±69.67) cpm/μg, respectively, all P<0.01］. CONCLUSION: Rosiglitazone prevents the development of hypertension and myocardial hypertrophy by inhibiting NADPH oxidase activity, reducing superoxide anion production and lowering AngII-induced hypertension.

HTML全文

参考文献(0)

施引文献

资源附件(0)