急性冠脉综合征患者PDCD4表达与血压变异性的相关性

张丽君

doi:10.12125/j.chj.202201008

急性冠脉综合征患者PDCD4表达与血压变异性的相关性

张丽君

Relationship between PDCD4 expression and blood pressure variability in patients with acute coronary syndrome

Li-jun ZHANG

摘要

摘要:
目的对急性冠脉综合征（ACS）患者程序性细胞死亡因子4（PDCD4）表达进行研究，并探讨其与血压变异性的相关性。
方法选择2019年1月～2021年1月联勤保障部队第904医院收治的270例ACS患者作为研究对象，根据Gensini评分结果将95例患者分到轻度病变组（0～30）分，102例患者分到中度病变组（31～60）分、73例患者分到重度病变组（>60）分。对所有研究对象进行连续24 h血压监测，记录24 h平均收缩压标准差（24 h mSBP-SD）、24 h平均舒张压标准差（24 h mDBP-SD）、白天平均收缩压标准差（d mSBP-SD）、白天平均舒张压标准差（d mDBP-SD）、夜间平均收缩压标准差（n mSBP-SD）和夜间平均舒张压标准差（n mDBP-SD）。采用实时荧光定量聚合酶链反应（qRT-PCR）检测CD4⁺T细胞PDCD4 mRNA表达水平，采用免疫印迹法检测CD4⁺T细胞PDCD4蛋白表达水平；采用Pearson法分析ACS患者PDCD4 mRNA和蛋白表达与血压变异性的相关性。
结果三组基线资料相比差异无统计学意义。血压变异性方面：中度病变组和重度病变组与轻度病变组比较、重度病变组与中度病变组比较，患者的24 h mSBP-SD、24 h mDBP-SD、d mSBP-SD、d mDBP-SD、n mSBP-SD与n mDBP-SD数值均明显增加（均P<0.01）。三组患者CD4⁺T细胞PDCD4 mRNA和蛋白表达等分子生物学数据方面，中度病变组和重度病变组与轻度病变组比较、重度病变组与中度病变组比较，患者CD4⁺T细胞PDCD4 mRNA和蛋白表达水平明显增加（均P<0.01）。Pearson法分析结果显示，ACS患者CD4⁺T细胞PDCD4 mRNA和蛋白表达水平均与24 h mSBP-SD、24 h mDBP-SD、d mSBP-SD、d mDBP-SD、n mSBP-SD及n mDBP-SD呈正相关（P<0.01）。
结论 PDCD4与ACS患者的血压变异性有关，可能用于评估患者病情严重程度。

Abstract:
AIM To study the expression of programmed cell death factor 4 (PDCD4) in patients with acute coronary syndrome (ACS) and its correlation with blood pressure variability.
METHODS A total of ACS 270 patients treated in our hospital from January 2019 to January 2021 were selected for this study. According to Gensini score, the 270 patients were divided into mild lesion group (0～30 points, 95 cases), moderate lesion group (31～60 points, 102 cases) and severe lesion group (> 60 points, 73 cases). Their blood pressure was monitored for 24 hours continuously, and 24h standard deviation of mean systolic blood pressure (24 h mSBP-SD), 24 h standard deviation of mean diastolic blood pressure (24 h mDBP-SD), standard deviation of mean systolic blood pressure daytime (d mSBP-SD), standard deviation of mean diastolic blood pressure daytime (d mDBP-SD), standard deviation of mean systolic blood pressure at night (n mSBP-SD) and standard deviation of mean diastolic blood pressure at night (n mDBP-SD) were recorded. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression level of PDCD4 mRNA in CD4+T cells, Western blotting was used to detect the expression level of PDCD4 protein in CD4+T cells and Pearson method was used to analyze the correlation between PDCD4 mRNA and protein expression and blood pressure variability in ACS patients.
RESULTS There was no significant difference between the three groups. In terms of blood pressure variability, the values of 24 h MSBP SD, 24 h mdbp SD, D MSBP SD, D mdbp SD, n MSBP SD and N mdbp SD were significantly increased in moderate lesion group, severe lesion group and mild lesion group (all P<0.01). In terms of molecular biological data such as the expression of PDCD4 mRNA and protein on CD4 + T cells in the three groups, the expression levels of PDCD4 mRNA and protein on CD4 + T cells in patients with moderate and severe lesions were significantly higher than those in patients with mild lesions and patients with severe and moderate lesions (all P<0.01). Pearson analysis showed that the expression levels of PDCD4 mRNA and protein in CD4 + T cells of ACS patients were positively correlated with 24 h MSBP SD, 24 h mdbp SD, D MSBP SD, D mdbp SD, n MSBP SD and N mdbp SD (all P<0.01).
CONCLUSION PDCD4 is related to blood pressure variability in patients with ACS and could be used to assess the severity of patients’ condition.

HTML全文

参考文献(18)

施引文献

资源附件(0)