Abstract: Vascular endothelial cells play an important role in regulating vascular function, maintaining cardiovascular system homeostasis and vascular remodeling. Recent studies have confirmed that mitochondrial autophagy, as a selective autophagy, is closely related to the homeostasis and oxidative stress reaction of vascular endothelial cells. Endoplasmic reticulum (ER) is an important subcellular organelle that maintains cell structure and function, and participates in many physiological activities of cells. Endoplasmic reticulum stress can induce autophagy and affect the morphology and function of mitochondria in a variety of ways. Studies have shown that endoplasmic reticulum stress plays an important role in vascular injury related diseases such as atherosclerosis. This review focuses on the relationship between endoplasmic reticulum stress and mitochondrial autophagy and their effects on the function of vascular endothelial cells, in order to provide reference for further research on the molecular mechanism of mitochondrial autophagy and endoplasmic reticulum stress regulating vascular endothelial cells