王小波, 赵汝舟, 蒋帅, 周详, 焦博, 张琳, 岳小娅, 刘勇, 余志斌. 一种增强缺氧耐力的新大鼠模型[J]. 心脏杂志, 2021, 33(1): 67-71. DOI: 10.12125/j.chj.202010020
    引用本文: 王小波, 赵汝舟, 蒋帅, 周详, 焦博, 张琳, 岳小娅, 刘勇, 余志斌. 一种增强缺氧耐力的新大鼠模型[J]. 心脏杂志, 2021, 33(1): 67-71. DOI: 10.12125/j.chj.202010020
    shu
    Xiao-bo WANG, Ru-zhou ZHAO, Shuai JIANG, Xiang ZHOU, Bo JIAO, Lin ZHANG, Xiao-ya YUE, Yong LIU, Zhi-bin YU. A noval rat model for enhancing hypoxia tolerance[J]. Chinese Heart Journal, 2021, 33(1): 67-71. DOI: 10.12125/j.chj.202010020
    Citation: Xiao-bo WANG, Ru-zhou ZHAO, Shuai JIANG, Xiang ZHOU, Bo JIAO, Lin ZHANG, Xiao-ya YUE, Yong LIU, Zhi-bin YU. A noval rat model for enhancing hypoxia tolerance[J]. Chinese Heart Journal, 2021, 33(1): 67-71. DOI: 10.12125/j.chj.202010020
    shu

    一种增强缺氧耐力的新大鼠模型

    A noval rat model for enhancing hypoxia tolerance

      摘要
    • 摘要:
        目的  探究一种新的快速提高缺氧耐力的大鼠模型。
        方法  采用小动物低压舱模拟不同的气压高度，观察SD大鼠4500 m适应10 h，返回地面1 h，然后在7620 m停留24 h后的存活率；同时观察SD大鼠在禁食24 h、48 h和72 h后，在7620 m停留24 h后的存活率，并检测禁食72 h大鼠心肌的自噬通量。
        结果  4500 m缺氧预适应可以提高7620 m停留24h大鼠的存活率（P < 0.05）；禁食24 h、48 h和72 h大鼠，在7620 m停留24h的存活率明显提高（P < 0.01）；禁食24 h联合4500 m预适应可使大鼠7620 m停留24h的存活率达到100%（P < 0.01）。此外，鼠龄影响7620 m停留24h的存活率，鼠龄较小的大鼠存活率更高（P < 0.01）。Western blot检测结果提示，禁食72 h大鼠心肌Beclin-1表达增加（P < 0.01），LC3-II与LC3-I之比增加（P < 0.01），p62蛋白表达降低（P < 0.01），表明心肌自噬通量增高。
        结论  禁食预处理可作为快速提高缺氧耐力的一种新型大鼠模型，心肌自噬增强与缺氧耐力提高的因果关系有待进一步研究。

       

      Abstract:
        AIM  To explore a new rat model for rapid improvement of hypoxia tolerance.
        METHODS  Small animal hypobaric chamber was used to simulate different barometric heights. The survival rate was obserred in SD rats after adapting to 4500 m for 10 h, returning to the ground for 1 h, and then staying at 7620 m for 24 h. The survival rate of SD rats after fasting for 24 h, 48 h, 72 h and staying at 7620 m for 24 h were also obserred and the myocardial autophagy flux of rats after fasting for 72 h was detected.
        RESULTS  The 4500 m hypoxic preconditioning improved the survival rate of rats staying at 7620 m for 24 h (P < 0.05); The survival rate of rats staying at 7620 m for 24 h after fasting for 24 h, 48 h and 72 h was significantly increased (P < 0.01); Fasting for 24 h combined with 4500 m preconditioning made the survival rate of rats staying at 7620 m for 24 h reached 100% (P < 0.01). In addition, the survival rate of rats staying at 7620 m for 24 h was affected by the age of rats, and the survival rate of younger rats was higher (P < 0.01). The results of Western blot showed that the expression of Beclin-1 (P < 0.01), the ratio of LC3-II to LC3-I increased (P < 0.01) and the expression of p62 protein decreased (P < 0.01) in the myocardium of rats after fasting for 72 hours, indicating that myocardial autophagy flux was inreased.
        CONCLUSION  Fasting preconditioning can be used as a new rat model to rapidly improve hypoxia tolerance, and the causal relationship between increased myocardial autophagy and hypoxia tolerance needs to be further studied.

       

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