冠心病患者血清趋化因子CXCR3及其配体MIG、IP-10表达水平与冠状动脉病变程度的关系

樊宗成; 潘文博; 钟万生; 梁有峰; 丁汝跃; 盛春梅; 刘圣好; 陈东; 程小兵

doi:10.12125/j.chj.202004065

冠心病患者血清趋化因子CXCR3及其配体MIG、IP-10表达水平与冠状动脉病变程度的关系

Expression levels of CXCR3, its ligand MIG and IP-10 and the degree of coronary artery disease in patients with coronary heart disease

摘要

摘要:
目的探讨冠心病患者血清趋化因子CXCR3及其配体MIG、IP-10表达水平与冠状动脉病变程度的关系。
方法选取2017年1月～2019年7月合肥第三人民医院心内科住院，并初步考虑拟诊冠心病患者200例，均行冠状动脉造影确定，将患者分为冠心病组（n=78）和非冠心病组(n=122)。使用ELISA检测方法检测患者血清趋化因子CXCR3及其配体MIG、IP-10表达水平，比较两组患者CXCR3、MIG、IP-10表达水平，并对冠心病各亚组间CXCR3、MIG、IP-10表达水平进行比较。对冠心病组和非冠心病组外周血T细胞亚群水平、外周血细胞Th1、Th2、Th1/Th2水平进行比较。对两组临床相关指标进行比较，分析冠心病与CXCR3及其配体MIG、IP-10间的相关性。
结果冠心病组患者CXCR3、MIG、IP-10表达水平显著高于非冠心病组（均P<0.01）。冠心病组患者中，一支病变患者36例，二支患者23例，多支患者19例。经过方差分析，冠心病各亚组间CXCR3、MIG、IP-10表达水平差异有统计学意义（均P<0.01）。冠脉病变程度越重，CXCR3及其配体MIG、IP-10表达水平越高。冠心病组CD3+、CD4+、CD4+/CD8+水平显著低于对照组（均P<0.01）。冠心病组Th1、Th1/Th2水平显著高于非冠心病组，Th2水平显著低于非冠心病组（P<0.01）。经过Logistic回归分析，CXCR3及其配体MIG、IP-10表达水平对冠心病有显著影响（P<0.01）。
结论 CXCR3及其配体MIG、IP-10表达水平可作为冠心病血清学炎症因子指标，可反映冠脉病变严重程度。

Abstract:
AIM Investigate the relationship between the expression levels of CXCR3, its ligand MIG and IP-10 and the degree of coronary artery disease in patients with coronary heart disease.
METHODS A total of 200 patients with coronary atherosclerotic heart disease who were hospitalized in the department of cardiology of our hospital from Jun 2017 to July 2019 were selected, and all of them underwent coronary angiography to determine whether they were coronary heart disease(CHD). The expression levels of CXCR3, MIG and IP-10 in serum of patients were detected by ELISA, and the expression levels of CXCR3, MIG and IP-10 in the two groups were compared, and the expression levels of CXCR3, MIG and IP-10 in each subgroup of CHD were compared. The levels of T cell subsets, Th1, Th2 and Th1/Th2 in peripheral blood of CHD group and non-CHD group were compared. The correlation between CHD, CXCR3 and its ligands, MIG, IP-10, were analyzed.
RESULTS Of the 200 patients, 78 were CHD patients and 122 were non-CHD patients. CXCR3, MIG and IP-10 expression levels of CHD patients were significantly higher in the CHD group than in the non-CHD group, with statistically significant differences (P<0.01). Among the 78 patients with CHD, 36 patients with one lesion, 23 patients with two lesions, and 19 patients with multiple lesions. After ANOVA, there were statistically significant differences in CXCR3, MIG and IP-10 expression levels among each CHD subgroup (P<0.01). The higher the degree of coronary artery lesions, the higher the expression levels of CXCR3 and its ligands MIG and IP-10. CD3+, CD4+, CD4+/CD8+ levels in the CHD group were significantly lower than those in the control group, with statistically significant differences (P<0.01). Th1 and Th1/Th2 levels in the CHD group were significantly higher than those in the non-CHD group, and Th2 levels were significantly lower than those in the non-CHD group, with statistically significant differences (P<0.01). According to Logistic regression analysis, the expression levels of CXCR3 and its ligands, MIG and IP-10 have a significant effect on CHD. (P<0.01).
CONCLUSION The expression levels of CXCR3 and its ligands MIG and IP-10 can be used as serological inflammatory cytokines of CHD which can reflect the severity of CHD, and deserve further clinical research.

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