血清miR-200a-3p与miR-30a-5p表达在心力衰竭诊断及预后评估中的价值

    The value of serum miR-200a-3p and miR-30a-5p expression in the diagnosis and prognosis evaluation of heart failure

      摘要
    • 摘要:
      目的  分析血清中微小核糖核酸200a-3p（miR-200a-3p）和微小核糖核酸30a-5p（miR-30a-5p）表达在心力衰竭（HF）诊断以及HF患者预后评估中的价值。
      方法  前瞻性选取内蒙古医科大学附属医院2018年4月～2020年4月期间收治的HF患者131例为研究对象（HF组），同时期健康体检志愿者131例为对照组；采用实时荧光定量PCR（qRT-PCR）测定血清中miR-200a-3p和miR-30a-5p表达水平；采用Spearman相关分析血清中miR-200a-3p和miR-30a-5p水平与HF患者病情严重程度的相关性；采用ROC曲线分析血清miR-200a-3p和miR-30a-5p对HF的诊断价值；采用Logistic回归分析HF患者预后的影响因素；采用Kaplan-Meier生存分析血清miR-200a-3p和miR-30a-5p表达水平与HF患者预后的关系。
      结果  与对照组相比，HF组血清中miR-200a-3p和miR-30a-5p表达水平显著升高，差异具有统计学意义（P<0.05）；轻度HF、中度HF和重度HF患者血清中miR-200a-3p和miR-30a-5p表达水平依次升高，差异具有统计学意义（P<0.05）；Spearman相关分析显示，血清中miR-200a-3p和miR-30a-5p表达水平与HF患者病情严重程度呈正相关（rs=0.442，rs=0.564，均P<0.05）；ROC曲线分析结果显示，miR-200a-3p和miR-30a-5p联合诊断HF较miR-200a-3p和miR-30a-5p单一指标诊断效果更好（P<0.05）；Logistic回归分析结果发现，NT-proBNP、LVEF、LVEDD、miR-200a-3p和miR-30a-5p是HF患者预后的影响因素（Wald χ2=4.321～128.193，P<0.05）；Kaplan-Meier生存分析结果显示，血清中miR-200a-3p和miR-30a-5p高表达患者3年生存率低于miR-200a-3p和miR-30a-5p低表达患者（均P<0.05）。
      结论  HF患者血清中miR-200a-3p和miR-30a-5p表达水平显著升高，二者与HF的发生及患者预后相关。

       

      Abstract:
      AIM To analyze the value of serum microribonucleic acid 200a-3p (miR-200a-3p) and microribonucleic acid 30a-5p (miR-30a-5p) expression in the diagnosis of heart failure (HF) and prognosis evaluation of HF patients.
      METHODS  prospective study, 131 HF patients admitted to Inner Mongolia Medical University Affiliated Hospital from April 2018 to April 2020 were selected as the study subjects (HF group), 131 healthy volunteers undergoing simultaneous physical examination were included as the control group; real-time fluorescence quantitative PCR (qRT-PCR) was applied to determine the expression levels of miR-200a-3p and miR-30a-5p in serum; Spearman correlation analysis was applied to analyze the correlation between serum levels of miR-200a-3p and miR-30a-5p and the severity of condition; receiver operating characteristic was applied to analyze the diagnostic value of serum miR-200a-3p and miR-30a-5p for HF; Logistic regression was applied to analyze the influencing factors of prognosis in HF patients; and Kaplan-Meier survival was applied to analyze the relationship between serum miR-200a-3p and miR-30a-5p expression levels and the prognosis of HF patients.
      RESULTS  Compared with the control group, the expression levels of miR-200a-3p and miR-30a-5p in the serum of the HF group were significantly increased, and the difference was statistically significant (P<0.05); The expression levels of miR-200a-3p and miR-30a-5p in the serum of patients with mild, moderate, and severe HF increased sequentially, and the differences were statistically significant (P<0.05); Spearman correlation analysis showed that the expression levels of miR-200a-3p and miR-30a-5p in serum were positively correlated with the severity of HF patients (rs=0.442, rs=0.564, all P<0.05); The ROC curve analysis results show that the combined diagnosis of miR-200a-3p and miR-30a-5p for HF is more effective than the single indicator diagnosis of miR-200a-3p and miR-30a-5p (P<0.05); Logistic regression analysis found that NT-proBNP, LVEF, LVEDD, miR-200a-3p and miR-30a-5p are prognostic factors for HF patients (Wald) χ2=4.321～128.193, P<0.05); The Kaplan Meier survival analysis results showed that the 3-year survival rate of patients with high expression of miR-200a-3p and miR-30a-5p in serum was lower than that of patients with low expression of miR-200a-3p and miR-30a-5p (both P<0.05).
      CONCLUSION The expression levels of miR-200a-3p and miR-30a-5p in the serum of HF patients are obviously increased, which are related to the occurrence and prognosis of HF.

       

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