Abstract: Myocardial ischemia reperfusion injury (MIRI) is a pathological state in which myocardial cells suffer from ischemia and hypoxia in a short time due to reduced blood perfusion and the injury is aggravated after reperfusion. The pathological process of MIRI mainly involves inflammatory reaction, oxidative stress injury, apoptosis, endothelial cell injury and calcium overload. JAK2-STAT3 signaling pathway, as the communication node of cell function, participates in many pathological processes such as ischemia, hypoxia, oxidative stress, cell growth, proliferation, apoptosis and inflammation. Many cytokines and drugs have been shown to play anti-inflammatory, anti-oxidative, anti-death and endothelial growth promoting roles through JAK2-STAT3 signaling pathway. This review focuses on the relationship between JAK2-STAT3 signaling pathway and the pathological mechanism of myocardial ischemia reperfusion injury, with a view to providing a new therapeutic target for myocardial ischemia reperfusion injury.