吴秋文, 王帆, 孟玮, 张烁. CAR和Cx45在房室传导阻滞大鼠房室结区的表达[J]. 心脏杂志, 2020, 32(2): 109-113. DOI: 10.12125/j.chj.201911051
    引用本文: 吴秋文, 王帆, 孟玮, 张烁. CAR和Cx45在房室传导阻滞大鼠房室结区的表达[J]. 心脏杂志, 2020, 32(2): 109-113. DOI: 10.12125/j.chj.201911051
    Qiu-wen WU, Fan WANG, Wei MENG, Shuo ZHANG. Expressions of coxsackievirus-adenovirus receptor and connexin45 in node tissues of rats with atrioventricular block[J]. Chinese Heart Journal, 2020, 32(2): 109-113. DOI: 10.12125/j.chj.201911051
    Citation: Qiu-wen WU, Fan WANG, Wei MENG, Shuo ZHANG. Expressions of coxsackievirus-adenovirus receptor and connexin45 in node tissues of rats with atrioventricular block[J]. Chinese Heart Journal, 2020, 32(2): 109-113. DOI: 10.12125/j.chj.201911051

    CAR和Cx45在房室传导阻滞大鼠房室结区的表达

    Expressions of coxsackievirus-adenovirus receptor and connexin45 in node tissues of rats with atrioventricular block

    • 摘要:
        目的  研究急性心肌梗死后房室传导阻滞(AVB)大鼠房室结区柯萨奇病毒-腺病毒受体(CAR)和缝隙连接蛋白(Cx)45定位和表达情况,了解缺血相关AVB阻滞的发生机制及房室结区的电生理特征。
        方法  选择SD大鼠,随机分为模型组(n =12) 和假手术(SH)组(n = 6)。模型组根据AVB持续时间不同分为AVB 2h组(n = 6)和AVB 4h组(n = 6)。结扎大鼠右冠状动脉建立AVB阻滞模型,分别于建模后2h和4h对模型组和SH组取材。对房室结区行连续切片,行免疫荧光标记检测CAR和Cx45的定位及表达情况。行Western blot定量分析各组房室结区CAR和Cx45蛋白的表达情况。
        结果  免疫荧光标记显示CAR和 Cx45主要定位于房室结,模型组(AVB 2 h和AVB 4 h组)与SH组相比,CAR和Cx45表达增加。Western blot显示AVB 2 h组CAR和 Cx45 表达增加,与SH组相比,蛋白表达差异有统计学意义(P< 0.05); AVB 4 h组相比于SH组蛋白表达增多,但无统计学意义;CAR和Cx45蛋白表达相关性分析显示,CAR和 Cx45的表达具有明显正相关(r = 0.703,P< 0.05)。
        结论  急性心肌梗死后AVB阻滞早期大鼠房室结区CAR和Cx45表达增加,且CAR和Cx45的表达呈正相关。

       

      Abstract:
        AIM   To study the expressions of the coxsackievirus-adenovirus receptor and connexin45 in node tissues of rats with atrioventricular block and to understand the precise pathogenesis accounting for ischemia-induced atrioventricular block and electrophysiological behavior of the AV node.
        METHODS   SD rats were randomly divided into two groups: an atrioventricular block model group (n = 12) and a sham operation group (SH) (n = 6). The model group was divided into an AVB 2 h group (n = 6) and an AVB 4h group (n = 6), according to the time of atrioventricular block. The atrioventricular block rat model was induced by ligating the right coronary artery. The model group and the SH group were treated at 2 h and 4h after establishment of the model. Serial sections from the node area were obtained. Immunofluorescence analyses were performed to evaluate the locations and expressions of CAR and Cx45 in the node area. The expressions of CAR and Cx45 were quantitatively analyzed by Western blot.
        RESULTS   Immunofluorescence staining showed that CAR and Cx45 were mainly located in the atrioventricular node and expressions of CAR and Cx45 increased in the model group (AVB 2 h and AVB 4 h group) compared with those in the sham operation group. Western blot showed that the expressions of CAR and Cx45 in the AVB 2 h group were significantly higher than those in the sham operation group (P< 0.05). In the AVB 4h group, the expressions of CAR and Cx45 increased compared with those in the sham operation group, but there was no significant difference. Protein expression correlation analysis of CAR and Cx45 indicated that there was a significant positive correlation between CAR and Cx45 expressions (P< 0.05).
        CONCLUSION   The expressions of CAR and Cx45 protein increase in the node area of atrioventricular block rats after acute myocardial infarction and there is a significant positive correlation between CAR and Cx45 protein expressions.

       

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