董栋, 陈励, 张斌, 余琳, 马进, 谢满江. 短期模拟失重可引起大鼠视交叉上核中枢时钟基因的表达异常[J]. 心脏杂志, 2019, 31(3): 315-319. DOI: 10.12125/j.chj.201902038
    引用本文: 董栋, 陈励, 张斌, 余琳, 马进, 谢满江. 短期模拟失重可引起大鼠视交叉上核中枢时钟基因的表达异常[J]. 心脏杂志, 2019, 31(3): 315-319. DOI: 10.12125/j.chj.201902038
    Dong DONG, Li CHEN, Bin ZHANG, Lin YU, Jin MA, Man-jiang XIE. Short-term simulated weightlessness causes abnormal expression of rat circadian clock gene[J]. Chinese Heart Journal, 2019, 31(3): 315-319. DOI: 10.12125/j.chj.201902038
    Citation: Dong DONG, Li CHEN, Bin ZHANG, Lin YU, Jin MA, Man-jiang XIE. Short-term simulated weightlessness causes abnormal expression of rat circadian clock gene[J]. Chinese Heart Journal, 2019, 31(3): 315-319. DOI: 10.12125/j.chj.201902038

    短期模拟失重可引起大鼠视交叉上核中枢时钟基因的表达异常

    Short-term simulated weightlessness causes abnormal expression of rat circadian clock gene

    • 摘要:
        目的  探讨1周模拟失重对大鼠生物钟中枢时钟基因表达影响。
        方法  采用尾悬吊后肢去负荷模型模拟太空微重力环境,48只雄性SD大鼠随机均分为对照(control,CON)组和尾悬吊(tail-suspension,SUS)组。两组大鼠在相同的光照环境下(08:00~20:00)饲养。蛋白印迹实验检测大鼠视交叉上核(suprachiasmatic nucleus,SCN)内时钟基因(Per2Bmal1)以及钙通道Cav1.2蛋白在不同时间点的表达水平,同时采用实时定量PCR技术检测不同时间点SCN中Per2Bmal1的转录水平。
        结果  与CON组相比,短期模拟失重引起大鼠SCN中时钟基因Per2Bmal1 mRNA转录和蛋白表达下降(P<0.05),且波动幅度发生显著降低。此外,与对照组相比,模拟失重使得SUS组大鼠SCN中Cav1.2蛋白表达发生了明显上升(P<0.05)。
        结论  短期模拟失重可引起大鼠时钟基因表达异常,这可能是模拟失重引起机体发生病理性改变的机制之一,也为重力变化信号直接转化为时间节律信号提供了直接的证据。

       

      Abstract:
        AIM  To evaluate the effects of one-week simulated microgravity on the expression of circadian rhythm in rats.
        METHODS  The tail suspension hindlimb unloading model was used to simulate a space microgravity environment. 48 male SD rats (aged 8 weeks) were randomly assigned to a control (CON) and a tail suspension (SUS) group (24 each). Rats in both groups were housed under the same light conditions (8:00-20:00). Western blotting was used to detect the expression levels of clock genes (Per2, Bmal1) and ion channels Cav1.2 protein in the suprachiasmatic nucleus (SCN) of rats at different time points. Real-time quantitative PCR was used to detect the transcription levels of Per2 and Bmal1 in SCN at different time points.
        RESULTS  Short-term simulated weightlessness caused a decrease in the transcription and protein expression of clock genes Per2 and Bmal1 in rat SCN (P < 0.05) and the fluctuation amplitude was significantly reduced. In addition, compared with that in the control group, simulated weightlessness caused a significant increase in Cav1.2 protein expression in SCN of SUS rats.
        CONCLUSION  Short-term simulated weightlessness can cause abnormal expression of rat clock genes, which may be one of the mechanisms that simulate pathological changes of the body caused by weightlessness. This finding provides direct evidence for the direct conversion of gravity-changing signals into time-ratio signals.

       

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