彭子殷, 杨鑫, 张煜珅, 纪奇峰, 王伟. 茶与咖啡源性水溶性多酚的HUVEC细胞内抗氧化活性实验[J]. 心脏杂志, 2019, 31(2): 139-143. DOI: 10.12125/j.chj.201812011
    引用本文: 彭子殷, 杨鑫, 张煜珅, 纪奇峰, 王伟. 茶与咖啡源性水溶性多酚的HUVEC细胞内抗氧化活性实验[J]. 心脏杂志, 2019, 31(2): 139-143. DOI: 10.12125/j.chj.201812011
    Zi-yin PENG, Xin YANG, Yu-shen ZHANG, Qi-feng JI, Wei WANG. Experimental study on intracellular antioxidant activity of water soluble polyphenols from tea and coffee[J]. Chinese Heart Journal, 2019, 31(2): 139-143. DOI: 10.12125/j.chj.201812011
    Citation: Zi-yin PENG, Xin YANG, Yu-shen ZHANG, Qi-feng JI, Wei WANG. Experimental study on intracellular antioxidant activity of water soluble polyphenols from tea and coffee[J]. Chinese Heart Journal, 2019, 31(2): 139-143. DOI: 10.12125/j.chj.201812011

    茶与咖啡源性水溶性多酚的HUVEC细胞内抗氧化活性实验

    Experimental study on intracellular antioxidant activity of water soluble polyphenols from tea and coffee

    • 摘要:
        目的  探讨茶叶和咖啡在常饮方式下水溶性多酚对细胞氧化损伤可能的保护和修护作用。
        方法  建立人脐静脉内皮细胞(HUVEC)、胃粘膜GES-1和胃癌SGC7901细胞的H2O2损伤细胞氧化应激模型,二氯荧光素二乙酸酯探针(DCFH-DA)实验检测细胞内活性氧簇(ROS)的水平,四甲基偶氮唑蓝(MTT)比色法测定细胞活力,每种细胞设阴性对照组和三种茶叶两种咖啡水溶性多酚的三个浓度梯度组,评价多酚提取物对细胞氧化损伤的保护和修护作用。
        结果  测试的三种茶叶和两种咖啡均具有HUVEC细胞内的抗ROS生成与抗细胞氧化损伤能力,活性随着浓度的增加而增强(P<0.01)。绿茶的抗氧化性能最强,其次是两种咖啡,最后是红茶与黑茶。此种细胞内抗氧化活性在GES-1和SGC7901细胞中得到重复;5种多酚类饮料均不具备修复氧化损伤细胞的能力。
        结论  茶和咖啡的水溶性抗氧化成分可以有效进入血管内皮和胃粘膜上皮细胞,发挥清除ROS和抗细胞氧化的能力,但对于已经氧化损伤的细胞不具备修复能力。

       

      Abstract:
        AIM  To investigate possible protective and repair effects of water-soluble polyphenols in tea and coffee on oxidative damage at conventional drinking concentration.
        METHODS  Oxidative stress models of H2O2 injury of mainly human umbilical vein endothelial cells (HUVEC) and other cells were established. DCFH-DA probe test was used to detect the reactive oxygen species (ROS) level in cells and tetramethylazolidazole blue colorimetric test (MTT) was used to determine the cell viability.
        RESULTS  The three types of tea and two types of coffee tested all had anti-ROS generation and anti-cell oxidative damage ability in HUVEC cells and the activity increased with the increase of concentration. Green tea had the strongest antioxidant properties, followed by the two types of coffee and then black tea and dark tea. This intracellular antioxidant activity was repeated in gastric mucosa GES-1 and gastric cancer SGC7901 cells. None of the five polyphenolic beverages had the ability to repair oxidative damaged cells.
        CONCLUSION  The water-soluble antioxidant components of tea and coffee at conventional drinking concentration can effectively enter vascular endothelial cells and gastric mucosal epithelial cells. These water-soluble antioxidant components have the ability to clear ROS and resist cell oxidation, but they not have the ability to repair cells that have been oxidized.

       

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