王南丁, 马士航, 王文杰, 刘兴林, 胡乔斌, 王新冰, 郝伟, 黄晓莉. 芪丹通脉片调节TLR4信号通路抑制巨噬细胞泡沫化的研究[J]. 心脏杂志, 2019, 31(4): 392-396. DOI: 10.12125/j.chj.201810070
    引用本文: 王南丁, 马士航, 王文杰, 刘兴林, 胡乔斌, 王新冰, 郝伟, 黄晓莉. 芪丹通脉片调节TLR4信号通路抑制巨噬细胞泡沫化的研究[J]. 心脏杂志, 2019, 31(4): 392-396. DOI: 10.12125/j.chj.201810070
    Nan-ding WANG, Shi-hang MA, Wen-jie WANG, Xing-lin LIU, Qiao-bin HU, Xin-bing WANG, Wei HAO, Xiao-li HUANG. Qidantongmai Tablets prevents macrophage-to-foam cell transformation through regulating TLR4 signaling pathway[J]. Chinese Heart Journal, 2019, 31(4): 392-396. DOI: 10.12125/j.chj.201810070
    Citation: Nan-ding WANG, Shi-hang MA, Wen-jie WANG, Xing-lin LIU, Qiao-bin HU, Xin-bing WANG, Wei HAO, Xiao-li HUANG. Qidantongmai Tablets prevents macrophage-to-foam cell transformation through regulating TLR4 signaling pathway[J]. Chinese Heart Journal, 2019, 31(4): 392-396. DOI: 10.12125/j.chj.201810070

    芪丹通脉片调节TLR4信号通路抑制巨噬细胞泡沫化的研究

    Qidantongmai Tablets prevents macrophage-to-foam cell transformation through regulating TLR4 signaling pathway

    • 摘要:
        目的  观察益气活血中药芪丹通脉片对氧化低密度脂蛋白(ox-LDL)诱导的RAW264.7细胞泡沫化进程、炎症指标及Toll样受体(TLR)4信号通路的影响。
        方法  培养RAW264.7细胞,随机分为对照组、ox-LDL组及ox-LDL +芪丹通脉片(QDTM)组,各组细胞干预后进行泡沫化诱导,分析各组细胞油红染色阳性面积,计算各组细胞泡沫化诱导率,收集细胞,ELISA法检测炎性因子肿瘤坏死因子(TNF)-α、白介素(IL)-6、C反应蛋白(CRP)表达水平,Real-time PCR及Western blot分析TLR4、分子核因子(NF)-κB表达水平。
        结果  与对照组相比,ox-LDL组细胞泡沫化诱导率、TNF-α、IL-6、CRP水平显著升高(P < 0.05),TLR4、NF-κB表达水平明显上调,QDTM干预后,ox-LDL+QDTM组细胞泡沫化诱导率及TNF-α、IL-6、CRP水平较ox-LDL组显著下调(P < 0.05),同时TLR4、NF-κB表达水平较ox-LDL组明显下降。
        结论  QDTM能抑制TLR4信号通路及炎症反应、降低RAW264.7细胞泡沫化诱导率,抑制巨噬细胞泡沫化。

       

      Abstract:
        AIM  To observe the effects of Qidantongmai Tablets (QDTMT) on the transformation process of RAW264.7 cells to foam cells induced by ox-LDL and the expression levels of inflammatory factors and the TLR4 signaling pathway.
        METHODS  RAW264.7 cells were cultured and randomly divided into a control group, an ox-LDL induction group, and an ox-LDL induction + QDTMT group. The area of oil red positive expressions in each group was analyzed and the ratio of oil red O positive area/cell total area was computed after macrophage-to-foam cell transformation. After collection of the cells, the expression levels of inflammatory factors TNF-, IL-6 and CRP were detected by ELISA, and the expression levels of TLR4 and NF-κB factors were detected by real-time PCR and Western blot.
        RESULTS  Compared with those in the control group, the rate of oil red O positive area/cell total area and the expression levels of TNF-α, IL-6 and CRP in the ox-LDL induction group were significantly increased (P < 0.05) and at the same time, the expression levels of TLR4 and NF-κB factors were also significantly increased. However, after the QDTMT intervention, transformation rate and expression levels of TNF-α, IL-6 and CRP in the ox-LDL + QDTM group were significantly decreased (P < 0.05) when compared with those in the ox-LDL group and the expression levels of TLR4 and NF-κB were also significantly decreased.
        CONCLUSION  Inflammation plays an important role in the process of atherosclerotic plaque vulnerability. Qidantongmai Tablets inbihit the the TLR4 signaling pathway and significantly reduce the expression levels of inflammatory factors, the rate of oil red O positive area/cell total area, and prevents macrophage-to-foam cell transformation.

       

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