肠道菌群多样性改变在NAFLD诱发心血管疾病中的重要作用

王玉珍; 洪小卫; 尹涛; 刘海涛

doi:10.12125/j.chj.201810058

肠道菌群多样性改变在NAFLD诱发心血管疾病中的重要作用

Changes in diversity of gut microbiota play an important role in cardiovascular disease induced by nonalcoholic fatty liver disease

摘要

摘要:
目的探讨肠道菌群多样性的改变在非酒精性脂肪性肝脏疾病（NAFLD）诱发心血管疾病中的重要作用。
方法通过高脂饲料喂养的方式建立NAFLD的模型（模型组），并以正常饲料喂养的大鼠作为对照组，运用HE染色观察模型组与对照组肝脏组织形态的改变；通过Western blot检测两组肝脏细胞内SREBP-1c蛋白水平的改变；通过ELISA试剂盒检测两组血清中甘油三酯、总胆固醇、三甲胺以及氧化三甲胺的水平；运用16s rDNA高通量检测模型组与对照组肠道菌群结构的差异改变。
结果通过高脂饲料的喂养，建立了NAFLD的大鼠模型，与对照组相比，模型组肝脏细胞内脂肪过量堆积，炎性浸润增加（P < 0.05）；血清中甘油三酯和总胆固醇水平显著增加（P < 0.05），三甲胺以及氧化三甲胺的水平显著升高（P < 0.05）；肠道菌群多样性结构发生改变，乳酸杆菌的数量及比例显著降低（P < 0.05）。
结论 NAFLD能够引起肠道菌群的多样性发生改变，肠道菌群的多样性发生改变可能参与了NAFLD诱发心血管疾病的病理过程。

Abstract:
AIM To explore the changes in the diversity of gut microbiota in patients with non-alcoholic fatty liver disease and further clarify the important role or roles of the changes in the diversity of gut flora in nonalcoholic fatty liver disease (NAFLD) induced cardiovascular diseases rats.
METHODS The model of NAFLD was established by high-fat feeding and the changes of liver morphology in the model group and the control group were observed by HE staining technique. SREBP-1c levels were detected by Western blot in liver cells of both groups. Serum triglyceride, total cholesterol, trimethylamine and trimethylamine oxide were measured by an ELISA kit. The difference of gut flora structure between NAFLD group and control group was detected by high-throughput 16s rDNA.
RESULTS Compared with control group, the excessive accumulation of fat in the liver cells and inflammatory infiltration increased in the rat model group. Compared with the control group, serum levels of triglycerides and total cholesterol in the model group were significantly increased. In the model group, the diversity of gut flora was significantly altered, and the number and proportion of lactobacillus were significantly reduced.
CONCLUSION NAFLD causes changes in the diversity of gut flora, which may be a key link in the induction of cardiovascular disease by NAFLD.

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