依那普利抑制同型半胱氨酸致血管内皮损伤的保护作用

周岩芬; 蒋伟; 张敏; 赵连友; 侯杰军; 陈金锋; 范虹; 于小勇; 刘超峰; 雷忠义

doi:10.12125/j.chj.201806041

依那普利抑制同型半胱氨酸致血管内皮损伤的保护作用

Protective effect of enalapril to Hcy-related vascular endothelia injury

摘要

摘要:
目的探讨依那普利对同型半胱氨酸（Hcy）引起的血管内皮细胞损伤的保护作用。
方法取大鼠主动脉内皮细胞，培养并传代备用，进行血管内皮细胞药物保护实验。分为正常对照组（正常组）：不加Hcy及依那普利；损伤组：给予Hcy 2000 μmol/L；干预组：在给予Hcy 2000 μmol/L的同时再给予依那普利，依那普利的浓度不同又分为干预A、B、C、D和E组，其依那普利的浓度依次为10，20,40,80和160 nmol/L。内皮细胞的鉴定利用因子VIII间接免疫荧光；利用光学仪器进行血管内皮细胞形态学观察；测定各组细胞乳酸脱氢酶（LDH）的活性；利用CCK8试剂检测各组血管内皮细胞活力；利用细胞计数板测定活细胞百分率。
结果：① Hcy损伤组血管内皮细胞呈现出细胞损伤形态学改变；② 各组细胞LDH活性与正常对照组(100%)比较分别为：损伤组细胞LDH活性为（157 ± 17）%，干预C组（依那普利浓度40 nmol/L）细胞活性为（126 ± 7）%。干预C组较损伤组LDH活性显组降低（P<0.01）;③血管内皮细胞活力：正常组细胞活力定为 100%，损伤组为（52 ± 6）%，干预C组为（75 ± 6）%。干预C组细胞活力较损伤组相比细胞活力显著升高（P<0.01）；④ 活细胞百分率测定，与正常对照组（98 ± 1）%比较，损伤组活细胞比例（72 ± 4）%显著降低（P<0.01）；干预C组活细胞比例为(87 ± 3)% 较损伤组显著增加（P<0.01）。
结论依那普利可改善Hcy对血管内皮细胞的损伤，具有保护作用。

Abstract:
AIM To investigate the protective effect of enalapril on Hcy-induced vascular endothelial cell injury.
METHORDS Vascular endothelial cells were cultured and subcultured, divided into three groups: normal control cells (without drugs and Hcy), cells in an injury group (without drugs, only Hcy) and cells in the administration group (enalapril, plus Hcy). The dose of homocysteine is 2000μmol/L. The dosages of enalapril in the drug A group were 10 nmol/L, the drug in the B group were 20 nmol/L, the drug in the C group were 40 nmol/L, the drug in the D group were 80 nmol/L and the drug in the E group were 160 nmol/L. First, endothelial cells were identified by using factor VIII. Second, each group was morphological evaluated. Third, LDH activity detection was performed on each group. Fourth, CCK8 reagents were used to detect vitality of vascular endothelial cells in each group. Fifth, a cell count board was used to determine the percentage of living cells.
RESULTS ①The vascular endothelial cells of the injury group and the drug group were morphologically injured; ②The percentage of cell activity was（157 ± 17）% in the Hcy injury group, （126 ± 7）% in the drug C group. Compared with the Hcy injury group, the LDH activity was significantly decreased in the drug group（P < 0.01）; ③ Result of vascular endothelial cell activity showed that the Hcy injury group was（52 ± 6）%, the drug C group was（75 ± 6）%. Compared with the injury group, enalapril signifcantly increased the cell activity; ④ Regarding percentage of living cells, the normal group was（98 ± 1）%, the Hcy injury group was（72 ± 4)%, the drug C group was（87±3）%, and the latter increased significantly (P < 0.01).
CONCLUSION Damage of vascular endothelial cells by Hcy can be improved by enalapril.

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