Effects of ischemie-reperfusion on combined date of QRS complex waves of electrocardiogram, myocardial enzymes, nitric oxide synthase and malondialdehyde during ischemia-reperfusion in rabbits

AIM To explore the changes of QRS combined data and myocardial enzymes during ischemia-reperfusion in rabbits and to observe the changes of the nitric oxide synthase (NOS) and malondialdehyde (MDA). METHODS The models of ischemia-reperfusion were made by ligating the left circumflex territory for 10, 20, 30, 40 min and then reperfusing them for 60 min. The sham-operated control was designed. The changes of the QRS combined date were observed. The activity of serum myocardial enzymes and myocardial NOS and MDA were measured. RESULTS ①QRS combined date: The QRS combined date was significantly higher in the group of ischemia 40 min than that of the sham-operated control, and that of the groups of ischemia 10 min and 20 min. ②Serum myocardial enzymes: The activity of serum aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) significantly increased in ischemia 40 min than those in other groups. Serum creatine kinase (CK) was markedly high except the sham-operated control. The activity of serum CK-MB was significantly high in the groups of ischemia 30 min and 40 min. ③NOS: The activities of myocardial NOS in the group of ischemia 40 min was significantly higher than that of the sham-operated control. ④MDA: The myocardial MDA content in the group of ischemia 40 min was significantly higher than that of the sham-operated control. CONCLUSION LDH, CK-MB, NOS, MDA and the QRS combined date rise significantly in the group of ischemia 40 min.