Effect of TNF-α on cultured neonate cardiomyocytes

AIM:To explore the effects of TNF-α of different concentrations on cardiomyocyte vitality, protein synthesis, AngII secretion and AT1 receptor expression. METHODS: Cardiomyocytes were primarily cultured in Sprague Dawley neonate rats. Protein and cell vitality of myocytes were determined, respectively, by BCA method and MTT and LDH assay. Content of AngII in cell culture fluid was detected by enzyme-linked immunosorbent assay and expression alteration of AT1 receptor in myocytes was examined by immunocytochemical assay. RESULTS: TNF-α promoted AngII secretion in cardiomyocytes in a concentration-dependent manner. Low concentrations of TNF-α increased cell viability and promoted protein synthesis and AT1 receptor expression in myocytes. High concentrations of TNF-α induced myocyte injury. CONCLUSION: TNF-α promotes the secretion of AngII in myocytes and is involved in cardiac remodeling.