Relationship between expression of myocardial sodium-hydrogen exchanger 1 mRNA and endothelin and serum collagen in patients with chronic heart failure

AIM:To investigate the relationship between expression of myocardial sodium-hydrogen exchanger 1 (NHE1) mRNA and endothelin (ET-1) procollagen type I (PICP) and procollagen type Ⅲ (PIIINP) in patients with chronic heart failure (CHF). METHODS: Expressions of NHE1mRNA in 35 heart failure patients were detected using real-time fluorescence quantitative PCR (FQ-PCR). Plasma levels of ET-1, PⅠCP and PIIINP were tested by special radioimmunoassay. Left atrial diameter and left ventricular ejection fraction were measured by Doppler echocardiography. RESULTS: Using the real-time fluorescence quantitative PCR method, △Ct value of class I group, class II group and class Ⅲ group were 6.29±0.66, 5.01±0.60, and 4.40±0.74, respectively. Expressions of myocardial sodium-hydrogen exchanger 1 mRNA in group of class II and class III were significantly higher than in class I group (P<0.01). The expression of myocardial sodium-hydrogen exchanger 1 mRNA in group of class III was significantly higher than in group of class II (P<0.05). Plasma levels of ET-1, PICP and PIIINP in CHF were significantly higher than those in control group and the levels of ET-1, PICP and PIIINP were upregulated significantly by cardiac function classes according to the New York Heart Association. ET-1, PICP and PIIINP were negatively correlated with NHE1 mRNA △Ct and NHE1 mRNA △Ct was negatively correlated with ET-1. CONCLUSION: Change of expression of NHE1 mRNA in patients with CHF is possibly one of the molecule mechanisms of myocardial remodeling and is related to the genesis and maintenance of heart failure. NHE1, ET-1, PⅠCP and PIIINP are positively related. NHE1 may play an important role in the occurrence and development of heart failure.