Effects of concentration and molecular weight of polyethyleneimine on heparin-coated modified polyvinyl chloride material

AIM：To study the effects of concentration and molecular weight of polyethyleneimine (PEI) on PEI-heparin-coated polyvinyl chloride (PVC) medical materials. METHODS: Using PEI-glutaraldehyde-heparin coating techniques, cardiopulmonary bypass (CPB) circuits were coated with heparin and then divided into nine groups by PEI concentrations and molecular weights. The content of the coated heparin was calculated by toluidine blue spectrophotometry. RESULTS: The combination of the amount of heparin on PEI-heparin-coated PVC medical material was affected by the concentration and molecular weight of PEI. The optimal concentration and molecular weight of PEI were 2 g/dl and 20 000, respectively. CONCLUSION: Heparin concentration on PEI-glutaraldehyde-heparin-coated PVC surface is increased by the concentration and molecular weight of PEI. The increase is to some degree positively related to the content of PEI. Too high or too low PEI concentrations are not conducive to the combination of heparin.