Effect of non-genomic mechanism of thyronine in promoting the differentiation of cardiomyocytes derived from mouse-induced pluripotent stem cells

AIM To investigate the non-genomic mechanism of thyroid hormone in the induction of miPS cells to differentiate into myocardial cells. METHODSWe cultivated miPS cells using the hanging drop method to culture and form embryoid bodies with thyroxine and triiodothyroacetic acid (which can competitively combine with thyroxine membrane receptor, Triac) under the same conditions to induce miPS cells to differentiate into myocardial-like cells. Ebs were cultured in three groups: control groups, T3 group and TH+Triac group. During different periods (on the 4th, 6th and 12th days) we observed the characteristics of differentiation under the inverted microscope. Immunohistochemical technique and Western blot method were used to detect interacting protein expression in different situations. RESULTSAccording to comparison of the three groups, in the thyroxine group not only the beating EB areas were greater than the control group and TH+Triac group, but miPS cells were also induced into myocardial-like cells, which were more powerful than in the control group. CONCLUSIONThe non-genomic mechanism of thyroid hormone infuences miPS cells to differentiate into myocardial cells, possibly via the JNKs signaling pathway.