Effect of probucol on advanced glycosylation end product-induced dysfunction in cardiac microvascular endothelial cells

AIM:To observe the effect of probucol on the iNOS expression induced by advanced glycosylation end products (AGEs) in cardiac microvascular endothelial cells. METHODS: Primary cardiac microvascular endothelial cells were cultured in vitro. After treatment with AGEs (100 mg/L) and probucol (5 μmol/L, 10 μmol/L, 20 μmol/L), NO, iNOS and ROS protein expressions in all groups were detected and compared between groups. RESULTS: Compared with AGEs group, NO and iNOS protein expression increased and ROS protein expression decreased in groups treated with probucol. CONCLUSION: Probucol is able to partially recover AGE-induced dysfunction in cardiac microvascular endothelial cells.