Knockdown of GPM6B represses expression of smooth muscle cell specific genes

AIM To determine the effects of the Glycoprotein M6B (GPM6B) gene on mesenchymal cells differentiating into smooth muscle cells. METHODS Infected sh-GPM6B cells were used as treatment group cells, and sh-Scramle cells were used as a control group. sh-GPM6B plasmids and tool plasmids were used to pack lentivirus in the 293T cell line. The virus was used to infect C3H10T1/2 cells into a sh-GPM6B stable transferring cell line. A fluorescent microscope was used to detect transfer efficiency and Western blot was used to detect interference efficiency. After incubation with TGF-β1 for 3 days in control cells and sh-GPM6B cells, the expression and transcription levels of smooth muscle cell specific marker proteins (SM-MHC and α-SMA) were detected by Western blot and q-PCR. RESULTS Compared with the sh-Scramble control group cells, expression levels of GPM6B in the sh-GPM6B treatment group cells infected with lenti-sh-GPM6B virus decreased to 40-70%. Compared with the sh-Scramble control group cells, the expression levels of SM-MHC and α-SMA proteins in the sh-GPM6B treatment group cells was significantly decreased (P<0.01). The transcription levels of SM-MHC and α-SMA mRNA were significantly decreased (P<0.01). CONCLUSION Knockdown of GPM6B represses mesenchymal cells differentiating into smooth muscle cells induced by TGF-β1.