AIM To investigate the effects of Danshen Dripping Pill (DDP) on coronary microvascular dysfunction (CMD) and myocardial remodeling by regulating the high mobility group box 1 (HMGB1)/receptor for advanced glycation end products (RAGE) signaling pathway.
METHODS Thirty SD rats were randomly assigned to sham surgery, model and DDP treatment groups (n=10 per group). CMD models were induced by sodium laurate injection and the treatment group was given 4 weeks of DDP intervention. Cardiac tissue histopathology, ELISA and transmission electron microscopy were used to evaluated the effects of DDP on CMD and myocardial remodeling, and changes in inflammatory factors and oxidative stress-related indicators were measured. We also analyzed the HMGB1/RAGE signaling pathway using qPCR and Western blot.
RESULTS DDP significantly improved myocardial pathological changes in CMD model rats and reduced inflammation and oxidative stress levels. Compared with those in the model group, DDP decreased myocardial cell degeneration, inflammatory cell infiltration and fibrosis, significantly down-regulated expressions of HMGB1, RAGE（P<0.05） and related inflammatory factors IL-1β and TNF-α (P<0.01), suppressed oxidative stress in cardiac tissues, reduced MDA expression and enhanced activities of antioxidant enzymes SOD and GSH-Px (P<0.05).
CONCLUSION DDP effectively ameliorates pathological changes in CMD and myocardial remodeling by modulating the HMGB1/RAGE signaling pathway, reducing inflammation and oxidative stress and restoring cardiac structure and function. This provides new insights and scientific basis for its clinical application in coronary heart disease treatment.
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