LIANG Jia-xing, LIANG Jun, LIANG Xue-feng, ZHANG Xin, KONG Si-han, GAO Hui, LI Juan, YANG Yan-ling. U-50488H inhibits hypoxia-induced primary pulmonary artery smooth muscle cells mitophagy[J]. Chinese Heart Journal, 2024, 36(6): 621-626. DOI: 10.12125/j.chj.202402046
    Citation: LIANG Jia-xing, LIANG Jun, LIANG Xue-feng, ZHANG Xin, KONG Si-han, GAO Hui, LI Juan, YANG Yan-ling. U-50488H inhibits hypoxia-induced primary pulmonary artery smooth muscle cells mitophagy[J]. Chinese Heart Journal, 2024, 36(6): 621-626. DOI: 10.12125/j.chj.202402046
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    U-50488H inhibits hypoxia-induced primary pulmonary artery smooth muscle cells mitophagy

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      • Abstract
    • AIM To explored the effect of hypoxia on pulmonary artery smooth cells mitophagy, and the role of U-50488H, a kappa opioid receptor agonist, in this process.
      METHODS Primary pulmonary artery smooth muscle cells were cultured, and randomly grouped and treated with hypoxia and/or U-50488H, the mitophagy associated protein and mRNA level, such as Bnip3 and Pink1 were examined.
      RESULTS PASMCs Bnip3 and Pink1 level were significantly elevated after hypoxia for 24 h (P<0.05), U-50488H inhibited Pink1 expression (P<0.01), ABT-737, a mitophagy agonist combined hypoxia markedly elevated PASMCs mitophagy level (P<0.01), which was reversed when cells were treated with U-50488H (P<0.01).
      CONCLUSION PASMCs mitophagy level is noticeable elevated after 24 h hypoxia, κ-opioid receptor activation with U-50488Hinhibits mitophagy associated protein transcription and translation.
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