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Volume 34 Issue 3
May  2022
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Article Contents
Dan-dan SONG, Guang-yu HU, Fu-yang ZHANG, Feng GAO, Fang-fang SUN, Zhe CUI, Ling TAO, Yan-bin SONG. Branched chain amino acid aminotransferase-1 regulates adipose-derived mesenchymal stem cell survival via P53 signaling.[J]. Chinese Heart Journal, 2022, 34(3): 256-261. doi: 10.12125/j.chj.202111123
Citation: Dan-dan SONG, Guang-yu HU, Fu-yang ZHANG, Feng GAO, Fang-fang SUN, Zhe CUI, Ling TAO, Yan-bin SONG. Branched chain amino acid aminotransferase-1 regulates adipose-derived mesenchymal stem cell survival via P53 signaling.[J]. Chinese Heart Journal, 2022, 34(3): 256-261. doi: 10.12125/j.chj.202111123

Branched chain amino acid aminotransferase-1 regulates adipose-derived mesenchymal stem cell survival via P53 signaling.

doi: 10.12125/j.chj.202111123
  • Received Date: 2021-11-29
  • Accepted Date: 2022-03-28
  • Rev Recd Date: 2022-03-28
  • Publish Date: 2022-05-26
  •   AIM  To determine the regulatory role of branched chain amino acid aminotransferase-1 (BCAT1) in the survival of adipose-derived mesenchymal stem cells (ADSCs).   METHODS  ADSCs were isolated from white adipose tissues of male Sprague Dawley rats. BCAT1 expression was knocked down or over-expressed in ADSCs by adenovirus transfection and ADSC apoptosis was induced by hydrogen dioxide stimulation. ADSC apoptosis was evaluated by CCK-8 cell viability assay, cleaved caspase-3 expression, and TUNEL staining. The activity of P53 was suppressed by the specific inhibitor PFT-1α in control or BCAT1 silencing ADSCs.   RESULTS  BCAT1, rather than BCAT2, was the predominant subtype expressed in ADSCs. Silencing of BCAT1 exacerbated, whereas BCAT1 over-expression ameliorated hydrogen dioxide-induced ADSC apoptosis. The downstream targets of P53 were significantly increased in BCAT1 knock-downed ADSCs when stimulated by hydrogen dioxide. Inhibition of P53 by its specific inhibitor PFT-1α reversed the adverse impact of BCAT1 silencing on ADSC survival.   CONCLUSION  BCAT1 regulates ADSC survival via a P53-dependent manner, revealing that BCAT1 is a promising target to enhance ADSC survival and their cardioprotective efficacy.

     

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