Study on myocardial protective effect of mulberry leaves extracton in diabetic mice

  AIM  To explore the myocardial protective effect of mulberry leaf extract (MLE) on diabetic mice based on oxidative stress.

  METHODS  Diabetic mice were induced by streptozotocin (STZ) and high glucose and lipid diet (HFD). Metformin (Met, 200 mg/kg) was used as positive control. MLE (100 mg/kg, 200 mg/kg, 400 mg/kg) was given to diabetic mice by gavage respectively. After 12 weeks of intervention, the changes of body weight, blood glucose, glycosylated serum protein, blood lipid, liver and kidney function, heart function and myocardial structure and oxidative stress were detected.

  RESULTS  Compared with the normal group, the general condition of diabetic mice was obviously abnormal (P<0.01), the metabolism of glucose and lipid was disordered (P<0.01), the heart appeared obvious pathological structural changes, the cardiac function was significantly decreased (P<0.01), the accumulation of reactive oxygen species (ROS) was increased (P<0.01), the expression of SIRT1 and SOD was significantly decreased (P<0.01), and the level of oxidative stress was significantly increased (P<0.01); compared with the model group, 400 mg / kg mulberry leaf extract could significantly improve the glucose and lipid metabolism ability, cardiac function, pathological changes of myocardial tissue structure and the level of oxidative stress of diabetes mice (P<0.01).

  CONCLUSION  Mulberry leaf extract protects the cardiac function of diabetic mice, and the effect is related to the inhibition of oxidative stress in myocardial tissue.