AIM To investigate the protective effect of enalapril on Hcy-induced vascular endothelial cell injury.
METHORDS Vascular endothelial cells were cultured and subcultured, divided into three groups: normal control cells (without drugs and Hcy), cells in an injury group (without drugs, only Hcy) and cells in the administration group (enalapril, plus Hcy). The dose of homocysteine is 2000μmol/L. The dosages of enalapril in the drug A group were 10 nmol/L, the drug in the B group were 20 nmol/L, the drug in the C group were 40 nmol/L, the drug in the D group were 80 nmol/L and the drug in the E group were 160 nmol/L. First, endothelial cells were identified by using factor VIII. Second, each group was morphological evaluated. Third, LDH activity detection was performed on each group. Fourth, CCK8 reagents were used to detect vitality of vascular endothelial cells in each group. Fifth, a cell count board was used to determine the percentage of living cells.
RESULTS ①The vascular endothelial cells of the injury group and the drug group were morphologically injured; ②The percentage of cell activity was（157 ± 17）% in the Hcy injury group, （126 ± 7）% in the drug C group. Compared with the Hcy injury group, the LDH activity was significantly decreased in the drug group（P < 0.01）; ③ Result of vascular endothelial cell activity showed that the Hcy injury group was（52 ± 6）%, the drug C group was（75 ± 6）%. Compared with the injury group, enalapril signifcantly increased the cell activity; ④ Regarding percentage of living cells, the normal group was（98 ± 1）%, the Hcy injury group was（72 ± 4)%, the drug C group was（87±3）%, and the latter increased significantly (P < 0.01).
CONCLUSION Damage of vascular endothelial cells by Hcy can be improved by enalapril.
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