Association of ApoE and SLCO1B1 gene polymorphisms and genotypes with blood lipid levels in Gansu

  AIM   AIM To study the distribution characteristics of SLCO1B1 and ApoE genes in the Gansu region and the effects of genotypes on blood lipid levels.

  METHODS   A cross-sectional study was conducted in 386 patients in the Department of Cardiology of Gansu Provincial People's Hospital and polymerase chain reaction (PCR) was used to analyze gene polymorphisms. Blood lipid levels at admission were detected and the distribution characteristics of different genotypes were analyzed. The correlation between genetic polymorphisms and blood lipid levels at admission was investigated and the correlation between genotypes and blood lipid levels was analyzed by following blood lipid levels in January and June.

  RESULTS   The distribution of SLCO1B1 1a/1a genotype was statistically significant between males and females and the distribution of *1a and *1b alleles was statistically significant between males and females. Correlation analysis between genotypes and lipid levels found statistical differences in E2/E3, E2/E3, E3/E3, E3/E4 high-density lipoprotein (HDL) levels and in E3/E4 and E2/E2 low-density lipoprotein levels (LDL). High-density lipoprotein (HDL) levels were significantly different between ε2 carrier and E3/E3. Lipid level analysis in normal and abnormal ApoE genotype carriers found that E2/E2+E2/E3 were significantly different in triglyceride (TG) and E3/E3 and E3/E4+E4/E4 were significantly different in cholesterol (TC), triglyceride (TG) and low-density lipoprotein (LDL). Analysis of blood lipid levels in SLCO1B1 genotype carriers with normal and abnormal levels revealed that 1a/1a+1a/1b+1b/1b and 1a/15+1b/15+15/15 were significantly different in cholesterol (TC), triglyceride (TG) and low-density lipoprotein (LDL), respectively. Linear regression analysis in Apoe genotype revealed that E4/E4 was negatively correlated with E3/E3 and E2/E2 was positively correlated with E3/E3 in LDL. E4/E4 was positively correlated with E3/E3 in TG and E4/E4 was positively correlated with E3/E3 in LDL.

  CONCLUSION   The distribution of SLCO1B1 genotype was related to gender, ε4 allele carriers had higher blood lipids and *15 allele had better lipid-lowering effects.