The effect of rehmannia glutinosa oligosaccharide on vascular endothelial cell growth factor secreted by human adipose tissue-derived mesenchymal stem cells

AIM To isolate and culture human adipose tissue-derived mesenchymal stromal cells (hADMSCs) and to study the effect of rehmannia glutinosa oligosaccharide（RGO） on the secretion of vascular endothelial cell growth factor(VEGF) from ADMSCs. METHODS Human adipose tissue from patients receiving abdominal surgery was digested with 2.5 g/L collagenase typeⅠ, hADMSCs were separated and cells from sediment were cultured. The expression of CD44, CD105, CD45 and CD34 on ADMSCs were examined by immunocytochemical staining. ADMSCs were cultured in IMDM culture medium containing different concentrations (0，1，10，100，400 mg/L) of RGO. The effects of different concentrations of RGO on hADMSCs proliferation were measured by MTT colorimetry. At 72 hours after culture, ELISA was used to detect the level of VEGF in different culture mediums. RESULTS ADMSCs were identified by positive expression of CD44 and CD105 and negative expression of CD34 or CD45. According to MTT colorimetry’s results, there was little and insignificant difference in proliferation between groups of 1 mg/L and 10 mg/L and control group. Significant proliferation was seen in ADMSCs of groups of 100 mg/L and 400 mg/L (P<0.01). The contents of VEGF were (627.88±33.86), (655.50±40.29), (666.50±43.20), （843.50±53.05） and （722.88±51.34）ng/L, respectively. CONCLUSION RGO has significant effect on ADMSCs’s proliferation and can promote secretion of VEGF.