AIM To investigate whether intermittent hypoxic adaptation (IHA) can attenuate myocardial ischemia/reperfusion (I/R) injury by improving the ability of plasma membrane repair.
METHODS Small animal hypobaric chamber was used to simulate different barometric heights. Male Sprague Dawley rats were randomly divided into four groups: Sham, Sham+IHA, I/R, I/R+IHA. After 7 days of IHA, different treatments were used in each group. In I/R group, LAD was ligated for 30 minutes and then reperfusion for 12 hours. The myocardial ischemia/reperfusion injury was assessed with HE staining, echocardiography. The fluorescence intensity of mg-53 in myocardial tissue was detected by immunofluorescence assay. The levels of serum lactate dehydrogenase isoenzyme-1 (LDH-1) and creatine kinase isoenzyme-MB (CK-MB) were detected by enzyme-linked immunosorbent assay (ELISA). The expression of dysferlin, caveolin-3 and MG-53 proteinwere detected by Western Blot.
RESULTS There was no significant difference in all indexes between Sham and Sham+IHA. Compared with those in I/R group, the myocardial injury in I/R+IHA group was lighter, and the systolic function were significantly increased (P<0.01). Compared with those in IR group, the fluorescence intensity of MG-53 in I/R+IHA group was significantly increased (P<0.05), and the expression levels of dysfelin, MG-53 and caveolin-3 protein were significantly increased (P<0.05).
CONCLUSION IHA treatment can reduce myocardial I/R injury by improving the ability of plasma membrane repair in adult SD rats.
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