Melatonin Improves Ischemic Heart Failure by Activating SIRT1/Inflammatory Factors

  AIM  To investigate the effect of melatonin infused in paraventricular nucleuses (PVN) to ameliorate ischemic heart failure and its mechanism by activating the SIRT1 signaling pathway and regulating inflammatory factors in the PVN.

  METHODS  Osmotic mini-pumps were implanted in PVN for chronic infusion of melatonin and the rat ischemic heart failure model was established one week later. After four weeks, the indexes of echocardiography were measured, and the activity of SIRT1 and norepinephrine levels of proinflammatory factor IL-1β and anti-inflammatory factor IL-10 in the PVN were determined using Western blot and immunofluorescence. The expression of norepinephrine in peripheral blood was detected using ELISA kit.

  RESULTS  Melatonin infusion in the PVN increased the activity of SIRT1 and protein expression level of IL-10, reduced the expression level of IL-1β and NE and improved obviously the cardiac functions in rats.

  CONCLUSION  PVN infusion of melatonin ameliorates ischemic heart failure by activating the SIRT1 signaling pathway, regulating inflammatory factors in the paraventricular nucleuses and reducing peripheral sympathetic activity in ischemic heart failure rats.