AIM To observe the effects of morroniside on the expressions of angiopoietin 1 (Ang-1) and fibroblast growth factor 2 (FGF-2) in the infarcted myocardium of rats with acute myocardial infarction.
METHODS Twenty male Sprague-Dawley rats weighing 260-280g were selected and an AMI rat model was prepared by ligation of the left anterior descending coronary artery. The rats after successful modeling were randomly divided into a sham operation group, a model group, a low dose group of morroniside (45 mg/kg), a middle dose group of morroniside (90 mg/kg), and a high dose group of morroniside (180 mg/kg). From the first day after modeling, the rats were given morroniside regularly by gavage, and the sham operation and model groups were given the same amount of distilled water. The model rats were sacrificed 7 days after continuous administration and the protein expression levels of Ang-1 and FGF-2 in the infarcted myocardium were detected by Western blot.
RESULTS Compared with those in the sham operation group, the protein expression of Ang-1 in the model group was significantly increased (P < 0.01), while the expression of FGF-2 showed an increasing tendency, but there was no significant difference (P>0.05). Compared with those in the model group, the expression levels of both Ang-1 and FGF-2 in the high dose group of morroniside were significantly increased (P < 0.01, P < 0.05).
CONCLUSION Moroniside can up-regulate the expression of Ang-1 and FGF-2 in the infarcted myocardium of rats with acute myocardial infarction and promote angiogenesis.
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