Abstract: AIM: To investigate the effects of low-frequency pulsed magnetic fields (LF-PMFs) on the proliferation, cell cycle and migration of cardiac microvascular endothelial cells (CMECs). METHODS: Cells were randomly divided into four groups (control group, 1.0 mT group, 1.4 mT group and 1.8 mT group). CMECs (except for those in the control group) were exposed to triangular low-frequency pulsed magnetic fields (15 Hz) for 4 h every day and cultured for 3 days. RESULTS: After a 3-day intervention, 1.4 mT and 1.8 mT magnetic fields were found to have accelerated the proliferation ［(0.200±0.043)A vs.(0.159±0.037)A, (0.225±0.042)A, vs.(0.159±0.037)A, P<0.05］ and the migration (27.20±4.76 vs. 22.60±4.77; 33.80±3.19 vs. 22.60±4.77, P<0.05) of CMECs. The percentage of cells at the S+G2 phase increased, but no obvious changes were observed in the 1.0 mT group (P<0.05). CONCLUSION: LF-PMFs of 1.4 mT and 1.8 mT accelerate proliferation and migration of CMECs. The percentage of cells at the S+G phase increases.